Document Detail


Expression of DNA Methyltransferase Genes in Four-Cell Bovine Embryos Cultured in the Presence of Oviductal Fluid.
MedLine Citation:
PMID:  23020809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.
Authors:
D Barrera; E V García; F Sinowatz; G A Palma; M A Jiménez-Díaz; D C Miceli
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-30
Journal Detail:
Title:  Anatomia, histologia, embryologia     Volume:  -     ISSN:  1439-0264     ISO Abbreviation:  Anat Histol Embryol     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704218     Medline TA:  Anat Histol Embryol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina.
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