| Expression of DNA Methyltransferase Genes in Four-Cell Bovine Embryos Cultured in the Presence of Oviductal Fluid. | |
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MedLine Citation:
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PMID: 23020809 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression. |
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Authors:
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D Barrera; E V García; F Sinowatz; G A Palma; M A Jiménez-Díaz; D C Miceli |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-30 |
Journal Detail:
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Title: Anatomia, histologia, embryologia Volume: - ISSN: 1439-0264 ISO Abbreviation: Anat Histol Embryol Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-10-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7704218 Medline TA: Anat Histol Embryol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Blackwell Verlag GmbH. |
Affiliation:
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Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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