| Expression of Cytosolic Phospholipase A2 in Equine Endometrium during the Oestrous Cycle and Early Pregnancy. | |
| | |
MedLine Citation:
|
PMID: 20626674 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Phospholipase A2 (PLA2) is a key enzyme for biosynthesis of PGF2α. Real-time RT-PCR and immunohistochemistry were used to determine transcription and cellular distribution of cytosolic PLA2 (cPLA2) in the equine endometrium during the oestrous cycle and early pregnancy. Endometrial biopsy and blood samples were collected from cycling mares on days (d) 8, 11, 15 and 18 (oestrus) (Day 0 = Day of ovulation; n = 5 for each day) and from pregnant mares (n = 4) on d15. Except for mares on d18 and some cyclic mares (n = 2) on d15 with low progesterone (P(4) ) concentrations (<3.18 nm), P(4) concentrations were high. Cytosolic PLA2 was mainly localized in the luminal epithelium and stroma was negative. Cytosolic PLA2 expression was negatively correlated with P(4) concentration (r = -0.75, P < 0.001) and differed according to the stage of the oestrous cycle (P < 0.05). Cytosolic PLA2 expression was high during oestrus and declined to basal levels on d8 (P < 0.05). Thereafter, there was a trend for increased cPLA2 expression as luteal phase progressed. However, as P(4) dropped below 3.18 nm on d15, cPLA2 expression increased (P < 0.05). In pregnant mares, cPLA2 expression was not different from cyclic mares on d15 with high P(4) concentrations. However, it was lower than cyclic mares with low P(4) concentrations (p < 0.05). In conclusion, cPLA2 isoform, a member of the PLA2 family that controls PGF2α secretion, is highly expressed in the endometrium at the expected time of luteolysis. Progesterone may be an inhibitor for cPLA2 expression. During pregnancy, cPLA2 expression may be sufficient to initiate the cascade for PGF2α secretion and does not play a direct role in maternal recognition of pregnancy. |
| | |
Authors:
|
Mm Ababneh; H Ababneh; F Shidaifat |
Related Documents
:
|
18569224 - Preliminary investigations into the ethological relevance of round-pen (round-yard) tra... 16726434 - The effects of prostalene and alfaprostol as uterine myotonics, and the effect on postp... 3572884 - Effect of ovarian hormones on promotion of bactericidal activity by uterine secretions ... 9378914 - Interspecific and extraspecific pregnancies in equids: anything goes. 18569224 - Preliminary investigations into the ethological relevance of round-pen (round-yard) tra... 19007674 - The changing role of maternal thyroid hormone in fetal brain development. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Reproduction in domestic animals = Zuchthygiene Volume: 46 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-03-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
|
Languages: eng Pagination: 268-74 Citation Subset: IM |
Copyright Information:
|
© 2010 Blackwell Verlag GmbH. |
Affiliation:
|
Department of Veterinary Clinical Sciences Faculty of Veterinary Medicine, Department of Basic Veterinary Sciences, Jordan University of Science and Technology, Irbid, Jordan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Improving the landscape for prevention.
Next Document: Genistein Alters the Release of Oxytocin, Prostaglandins, Cortisol and LH during Insemination in Gil...