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Expression of Circulatory Dendritic Cells and Regulatory T-Cells in Patients with Different Subsets of Coronary Artery Disease.
MedLine Citation:
PMID:  21297489     Owner:  NLM     Status:  Publisher    
BACKGROUND:: Dendritic cells (DCs), regulators of innate and adaptive immunity, may play an important role in atherosclerosis. DC invasion was found in early atherosclerotic lesions. We aimed to characterize circulating DC gene expression in patients with different subsets of coronary artery disease (CAD). METHODS:: Peripheral blood mononuclear cells (PBMCs) were quantified using real-time PCR and FACS in patients with acute coronary syndrome (ACS: STEMI, n=35; NSTEMI, n=30) and stable CAD (6 months after stent implantation without progression, n=15), compared to controls (n=15). DCs and T-cells (TCs) were characterized using specific primers for CD1a (immature), CD86 (mature), CD123 (plasmacytoid), BDCA1 (myeloid), CD178 (activated TCs) and FOXP3 (regulatory TCs). To evaluate whether serum of patients with STEMI induces DC-differentiation, incubation of patient serum was performed. RESULTS:: CD86 was upregulated and CD1a downregulated in all patients with CAD (p<0.05). Patients with STEMI and NSTEMI showed a downregulation of CD1a compared to patients with stable CAD (p≤0.01). In contrast stable CAD patients had elevated CD178 levels compared to STEMI and NSTEMI patients (p≤0.04). In STEMI patients, FOXP3 was downregulated compared to controls (p<0.0001). Incubation of STEMI serum induced an upregulation of CD1a and CD86 in a human DC cell line. Coincubation with a blocking antibody for heat shock protein 60 (HSP60) inhibited this upregulation. CONCLUSIONS:: DCs are differentially regulated in patients with different subsets of CAD. Mature DCs are upregulated and immature DCs are downregulated in patients with CAD. Patients with STEMI show a significant down-regulation of regulatory TCs. Circulating HSP60 induces DC-differentiation in STEMI patients.
Sieglinde Kofler; Zeljka Sisic; Nataliya Shvets; Peter Lohse; Michael Weis
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-03
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  -     ISSN:  1533-4023     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Medizinische Klinik und Poliklinik I, *Klinische Chemie, University Hospital Grosshadern; Ludwig-Maximilians University; Munich, Germany.
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