| Expression of cell cycle-related genes with cytokine-induced cell cycle progression of primitive hematopoietic stem cells. | |
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MedLine Citation:
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PMID: 19788373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Primitive marrow lineage-negative rhodamine low and Hoechst low (LRH) stem cells isolated on the basis of quiescence respond to the cytokines thrombopoietin, FLT3L, and steel factor by synchronously progressing through cell cycle. We have now profiled the mRNA expression, as determined by real-time RT-PCR, of 47 hematopoietic or cell cycle-related genes, focusing on the variations in the cell cycle regulators with cycle transit. LRH stem cells, at isolation, showed expression of all interrogated genes, but at relatively low levels. In our studies, there was a good deal of consistency with regard to cell cycle regulatory genes involved in the G1/S progression point of LRH murine stem cells. The observed pattern of expression of cyclin A2 is consistent with actions at these phases of cell cycle. Minimal elevations were seen at 16 h with higher elevations at 24, 32, 40, and 48 h times encompassing S, G2, and M phases. CDK2 expression pattern was also consistent with a role in G1/S transition with a modest elevation at 24 h and more substantial elevation at 32 h. The observed pattern of expression of cyclin F mRNA with marked elevations at 16-40 h was also consistent with actions in S and G2 phases. Cyclin D1 expression pattern was less consistent with its known role in G1 progression. The alterations in multiple other cell cycle regulators were consistent with previous information obtained in other cell systems. The cycle regulatory mechanics appears to be preserved across broad ranges of cell types. |
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Authors:
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Peter J Quesenberry; Gerri J Dooner; Michael Del Tatto; Gerald A Colvin; Kevin Johnson; Mark S Dooner |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Stem cells and development Volume: 19 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-08 Completed Date: 2010-08-13 Revised Date: 2012-07-06 |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
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Languages: eng Pagination: 453-60 Citation Subset: IM |
Affiliation:
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Rhode Island Hospital and the Brown Medical School, Division of Hematology/Oncology, Department of Medicine, Providence, Rhode Island 02903, USA. pquesenberry@lifespan.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic
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pharmacology Animals Cell Cycle / drug effects, genetics Cell Lineage* Cells, Cultured Cyclin-Dependent Kinase 2 / genetics Cyclins / genetics Gene Expression Hematopoiesis* / drug effects, genetics Hematopoietic Stem Cells / cytology*, drug effects, metabolism* Male Membrane Proteins / pharmacology Mice Rhodamines Stem Cell Factor / pharmacology Thrombopoietin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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1 P20 RR025179-01/RR/NCRR NIH HHS; 7R01 HL073749-05/HL/NHLBI NIH HHS; P20 RR025179-01/RR/NCRR NIH HHS; R01 HL073749-05/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Cyclins; 0/Membrane Proteins; 0/Rhodamines; 0/Stem Cell Factor; 0/flt3 ligand protein; 9014-42-0/Thrombopoietin; EC 2.7.11.22/Cyclin-Dependent Kinase 2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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