Document Detail


Expression of CYP2M1, CYP2K1, and CYP3A27 in brain, blood, small intestine, and other tissues of rainbow trout.
MedLine Citation:
PMID:  9535744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of five constitutive forms of cytochrome P450 [(LMC1 (CYP2M1), LMC2 (CYP2K1), LMC3, LMC4, and LMC5 (CYP3A27)] in selected tissues from sexually immature 2-year old female and male rainbow trout (Oncorhynchus mykiss) were examined at the translational level by Western blot using polyclonal antibodies raised in rabbits against those purified trout hepatic P450s. Tissues examined were from brain, liver, muscle, blood, head kidney, trunk kidney, upper intestine, stomach, heart, and gonad (ovary or testis). The results showed that the liver was the major organ for expression of all the trout P450s studied. Trunk kidney was the secondary expression site except for LMC5. Selective translational expression of these P450 isoforms or similar proteins was observed for LCM1 and LMC5 in brain; for LMC2 and LMC5 in female upper intestine; and for LMC2 in blood plasma of the fish studied under the experimental and sampling conditions.
Authors:
I Cok; J L Wang-Buhler; M M Kedzierski; C L Miranda; Y H Yang; D R Buhler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  244     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-04     Completed Date:  1998-05-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  790-5     Citation Subset:  IM    
Affiliation:
Department of Agricultural Chemistry and Marine/Freshwater Biomedical Sciences Center, Oregon State University, Corvallis 97331, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aryl Hydrocarbon Hydroxylases*
Blotting, Western
Brain / enzymology
Cytochrome P-450 Enzyme System / analysis*,  blood,  classification
Female
Fish Proteins*
Intestine, Small / enzymology
Kidney / enzymology
Liver / enzymology
Male
Oncorhynchus mykiss*
Sex Factors
Steroid Hydroxylases / analysis*,  blood,  classification
Terminology as Topic
Tissue Distribution
Grant Support
ID/Acronym/Agency:
ES000210/ES/NIEHS NIH HHS; ES03850/ES/NIEHS NIH HHS; ES04766/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Fish Proteins; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/Steroid Hydroxylases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/CYP2K1 protein, Oncorhynchus mykiss

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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