| Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. | |
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MedLine Citation:
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PMID: 19850844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. METHODS: Thirty-three patients with dry eye (16 with and 17 without Sjögren's syndrome) and 15 control subjects were recruited. The concentrations of CXCL9, -10, and -11 in tears were measured with enzyme-linked immunosorbent assays. The correlation between chemokine levels and tear film and ocular surface parameters was analyzed. The expression of CXCL9, -10, -11, and CXCR3 in the conjunctiva was evaluated by using immunohistochemistry. Flow cytometry was performed to count CXCR3(+) cells and CXCR3(+)CD4(+) cells in the conjunctiva. RESULTS: The concentrations of CXCL9, -10, and -11 were 1,148 +/- 1,088, 24,338 +/- 8,706, and 853 +/- 334 pg/mL, in the patients with dry eye, and 272 +/- 269 (P = 0.01), 18,149 +/- 5,266 (P = 0.02), and 486 +/- 175 (P < 0.01) pg/mL in the control subjects, respectively. The concentrations significantly increased in tears of the patients with Sjögren's syndrome compared with those of the patients with non-Sjögren's dry eye (P < 0.05). CXCL10 levels correlated significantly with basal tear secretion, and CXCL11 levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy score, and goblet cell density (P < 0.05). Staining for CXCL9, -10, -11, and CXCR3 increased in patients with dry eye, especially in the patients with Sjögren's syndrome. Flow cytometry demonstrated an increased number of CXCR3(+) and CXCR3(+)CD4(+) cells in all the patients with dry eye. CONCLUSIONS: Expression of CXCL9, -10, -11, and CXCR3 increased in the tear film and ocular surface of patients with dry eye syndrome, especially in those with Sjögren's syndrome. CXCL11 levels correlated significantly with various tear film and ocular surface parameters. (ClinicalTrials.gov number, NCT00991679.). |
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Authors:
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Kyung-Chul Yoon; Chang-Soo Park; In-Cheon You; Hwan-Jun Choi; Kwang-Hoon Lee; Seong-Kyu Im; Hong-Yong Park; Stephen C Pflugfelder |
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Publication Detail:
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Type: Clinical Trial; Journal Article Date: 2009-10-22 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 51 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-19 Completed Date: 2010-02-17 Revised Date: 2012-01-23 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 643-50 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Center for Biomedical Human Resources at Chonnam National University, Chonnam National University Medical School and Hospital, Gwang-Ju, Korea. kcyoon@jnu.ac.kr |
| Data Bank Information | |
Bank Name/Acc. No.:
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ClinicalTrials.gov/NCT00991679 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged CD4-Positive T-Lymphocytes / metabolism Cell Count Chemokine CXCL10 / metabolism* Chemokine CXCL11 / metabolism* Chemokine CXCL9 / metabolism* Conjunctiva / metabolism* Enzyme-Linked Immunosorbent Assay Eye Proteins / metabolism Female Flow Cytometry Goblet Cells / cytology Humans Immunoenzyme Techniques Male Middle Aged Prospective Studies Receptors, CXCR3 / metabolism* Sjogren's Syndrome / metabolism* Tears / metabolism* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY011915-12/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CXCL10 protein, human; 0/CXCL11 protein, human; 0/CXCL9 protein, human; 0/CXCR3 protein, human; 0/Chemokine CXCL10; 0/Chemokine CXCL11; 0/Chemokine CXCL9; 0/Eye Proteins; 0/Receptors, CXCR3; 0/tear proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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