Document Detail

Expression of CXC chemokines and their receptors is modulated during chondrogenic differentiation of human mesenchymal stem cells grown in three-dimensional scaffold: evidence in native cartilage.
MedLine Citation:
PMID:  18333808     Owner:  NLM     Status:  MEDLINE    
Chemokines contribute to the maintenance of cartilage homeostasis. To evaluate the role of CXC chemokines CXCL8 (interleukin-8), CXCL10 (interferon-gamma-inducible protein-10), CXCL12 (stroma-derived factor-1) and CXCL13 (B-cell attracting chemokine-1) and their receptors, respectively CXCR1-2, CXCR3, CXCR4, and CXCR5, during chondrogenic differentiation of human mesenchymal stromal cells (h-MSCs), we used a well-defined in vitro model. Chondrogenic differentiation was analyzed on h-MSCs grown on hyaluronic acid-based biomaterial in the presence or absence of transforming growth factor-beta, and the expression and modulation of CXC chemokines and receptors were evaluated at different time points. Real-time polymerase chain reaction was performed to analyze their expression at the messenger ribonucleic acid (mRNA) level, and immunohistochemistry and enzyme-linked immunosorbent assay were used to evaluate their expression at the protein level. Human articular cartilage biopsies were used to evaluate chemokine and receptor expression in normal tissue. We found no expression of CXCR1, CXCR2, CXCR3, or CXCL10 at the mRNA level. CXCL8 mRNA was down-modulated, whereas at the protein level, we found greater release of this chemokine. CXCR4 and its ligand CXCL12 were down-modulated during chondrogenesis. By contrast, CXCR5 was up-regulated, whereas its ligand CXCL13 was lower. These data were also confirmed on human articular cartilage. These findings show that, during in vitro h-MSC chondrogenic differentiation, chemokine and receptor expression was specifically induced or repressed. This was in line with what the authors also found in normal articular cartilage, suggesting a role in differentiation and maturation of a cartilage-like structure in vitro and consequently the regulation of cartilage homeostasis.
Sandra Cristino; Anna Piacentini; Cristina Manferdini; Katia Codeluppi; Francesco Grassi; Andrea Facchini; Gina Lisignoli
Related Documents :
19790048 - Hypoxia-inducible factor 1alpha inhibits the fibroblast-like markers type i and type ii...
17396138 - S100a1 and s100b, transcriptional targets of sox trio, inhibit terminal differentiation...
18075818 - Analysis of human osteoarthritic connective tissue by laser capture microdissection and...
8798568 - Cytokine control of interstitial collagenase and collagenase-3 gene expression in human...
9676188 - Requirement for the vasa rna helicase in gurken mrna localization.
21986708 - Serum amyloid a is a growth factor for 3t3-l1 adipocytes, inhibits differentiation and ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  14     ISSN:  1937-3341     ISO Abbreviation:  -     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-03-12     Completed Date:  2008-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-105     Citation Subset:  IM    
Laboratory of Immunology and Genetics, Istituti Ortopedici Rizzoli, Bologna, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cartilage, Articular / cytology,  metabolism*
Cell Differentiation / drug effects,  physiology*
Chemokines, CXC / biosynthesis*
Chondrocytes / cytology,  metabolism*
Chondrogenesis / drug effects,  physiology*
Gene Expression Regulation / drug effects,  physiology
Homeostasis / drug effects,  physiology
Hyaluronic Acid
Mesenchymal Stem Cells / cytology,  metabolism*
RNA, Messenger / biosynthesis
Receptors, CXCR / biosynthesis*
Transforming Growth Factor beta / pharmacology
Reg. No./Substance:
0/Chemokines, CXC; 0/RNA, Messenger; 0/Receptors, CXCR; 0/Transforming Growth Factor beta; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Functional tissue-engineered valves from cell-remodeled fibrin with commissural alignment of cell-pr...
Next Document:  Proliferation, osteogenic differentiation, and distribution of rat bone marrow stromal cells in nonw...