Document Detail


Expression of CD44 splice variants in squamous epithelia and squamous cell carcinomas of the head and neck.
MedLine Citation:
PMID:  8691348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Splice variants of the adhesion molecule CD44 have been described as essential for the lymphatic spread of rat tumour cells and are claimed to be involved in the metastatic spread of several human tumours. Immunohistochemistry has been used to analyse the expression pattern of CD44 standard (CD44s) and variant (CD44v) isoforms in normal and dysplastic squamous epithelia, as well as in primary and metastatic squamous cell carcinomas (SCCs), which spread predominantly by way of the lymphatic system. Frozen sections of squamous epithelia and of squamous cell carcinomas were stained with a panel of monoclonal antibodies recognizing epitopes of CD44s as well as of the variant exons v5, v6, v7, v7-v8, and v10. The stratum basale and stratum suprabasale of squamous epithelia stained with all antibodies; the stratum spinosum stained with anti-CD44v5, anti-CD44v6, anti-CD44v7-8 and anti-CD44v10; the lower layers of the stratum corneum stained with anti-CD44v5. This expression profile was seen in epithelia of the lip, the tongue, the gingiva, the hard palate, the floor of the mouth, the buccal mucosa, and the pharynx. The same pattern of expression was also noted in dysplastic epithelia, but expression of the variant exons v7, v8, and v10 was significantly downregulated in primary squamous cell carcinomas and was not detected at all in the majority of metastasis-derived specimens. Expression of CD44v5 and CD44v6, on the other hand, was mainly unaltered. Thus, epithelial cell layers representing different stages of differentiation express distinct sets of CD44 variant isoforms, where especially exons v8-v10 might be required for the maintenance of the structural integrity of squamous epithelium. Downregulation of these exons on tumour cells could indicate that they are irrelevant for tumour progression or may even hamper infiltration of surrounding tissue or of lymphatics.
Authors:
C Herold-Mende; S Seiter; A I Born; E Patzelt; M Schupp; J Zöller; F X Bosch; M Zöller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  179     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-08-23     Completed Date:  1996-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  66-73     Citation Subset:  IM    
Affiliation:
University of Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Alternative Splicing
Antibodies, Monoclonal
Antigens, CD44 / genetics,  metabolism*
Antigens, Neoplasm / genetics,  metabolism*
Carcinoma, Squamous Cell / immunology*,  secondary
Epithelium / immunology
Female
Head and Neck Neoplasms / immunology*,  pathology
Humans
Immunoenzyme Techniques
Male
Middle Aged
Mouth / immunology
Pharynx / immunology
Precancerous Conditions / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD44; 0/Antigens, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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