Document Detail


Expression of CD44 molecules and CD44 ligands during human thymic fetal development: expression of CD44 isoforms is developmentally regulated.
MedLine Citation:
PMID:  7537537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has recently been recognized that CD44 comprises a large family of alternatively spliced forms. In the thymus, CD44 has been postulated to play an important role in immature T cell migration and maturation. In this paper, we have studied the expression of CD44 molecules and two CD44 ligands, hyaluronan (HA) and fibronectin (FN), during human thymic fetal development. We found that mAbs against all CD44 isoforms (A3D8 or A1G3) reacted with both thymic epithelial (TE) cells and thymocytes beginning at the time of initial colonization of the human thymus by hematopoietic stem cells at 8.2 weeks of fetal gestation. However, mAbs specific for splice variants of CD44 containing membrane-proximal inserts (11.24, 11.10 and 11.9) reacted only with terminally differentiated TE cells in and around Hassall's bodies beginning at 16-19 weeks of fetal gestation. Studies of differentiated versus undifferentiated TE cells in vitro confirmed the selective expression of CD44 variant isoforms on terminally differentiated TE cells. Expression of HA and FN was determined by fluorescence microscopy using either biotinylated-HA binding protein or an anti-FN mAb. We found that whereas FN was present throughout the human fetal thymus beginning at 8.2 weeks, HA was not present until 16 weeks of gestational age. These data demonstrate the differential expression of standard versus variant CD44 isoforms during thymic ontogeny and implicate CD44 interactions with ligands other than HA as important in the earlier stages of human thymus development.
Authors:
D D Patel; L P Hale; L P Whichard; G Radcliff; C R Mackay; B F Haynes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International immunology     Volume:  7     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-07     Completed Date:  1995-06-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  277-86     Citation Subset:  IM    
Affiliation:
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antigens, CD44
Carrier Proteins / biosynthesis*,  immunology*
Cell Differentiation
Cells, Cultured
Embryonic and Fetal Development / immunology
Fibronectins / biosynthesis*,  metabolism
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression Regulation, Developmental
Humans
Hyaluronic Acid / biosynthesis*,  metabolism
Infant, Newborn
Receptors, Cell Surface / biosynthesis*,  immunology*
Receptors, Lymphocyte Homing / biosynthesis*,  immunology*
Thymus Gland / cytology,  embryology*
Grant Support
ID/Acronym/Agency:
AR39162/AR/NIAMS NIH HHS; CA28936/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD44; 0/Carrier Proteins; 0/Fibronectins; 0/Receptors, Cell Surface; 0/Receptors, Lymphocyte Homing; 9004-61-9/Hyaluronic Acid

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