Document Detail


Expression of CD1d under the control of a MHC class Ia promoter skews the development of NKT cells, but not CD8+ T cells.
MedLine Citation:
PMID:  14530332     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although CD1d and MHC class Ia share similar overall structure, they have distinct levels and patterns of surface expression. While the expression of CD1d1 is known to be essential for the development of NKT cells, the contribution of CD1d1 to the development of CD8(+) T cells appears to be inconsequential. To investigate whether CD1d tissue distribution and expression levels confer differential capacity in selecting these two T cell subsets, we analyzed CD8 and NKT cell compartments in K(b)-CD1d-transgenic mice that lack endogenous MHC class Ia and CD1d, respectively. We found that MHC class Ia-like expression pattern and tissue distribution are not sufficient for CD1d to rescue the development of CD8(+) T cells, suggesting that unique structural features of CD1d preclude its active participation in selection of CD8(+) T cells. Conversely, cell type-specific CD1d surface density is important for the selection of NKT cells, as the NKT cell compartment was only partially rescued by the K(b)-CD1d transgene. We have previously demonstrated that increased CD1d expression on dendritic cells enhanced negative selection of NKT cells. In this study, we show that cell type-specific expression levels of CD1d establish a narrow window between positive and negative selection, suggesting that the distinct CD1d expression pattern may be selected evolutionarily to ensure optimal output of NKT cells.
Authors:
Honglin Xu; Taehoon Chun; Angela Colmone; Hanh Nguyen; Chyung-Ru Wang
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  171     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-07     Completed Date:  2004-01-06     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4105-12     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD1 / biosynthesis*,  genetics*
Antigens, CD1d
CD8-Positive T-Lymphocytes / cytology,  immunology*,  metabolism
Cell Differentiation / genetics,  immunology*
Epitopes, T-Lymphocyte / metabolism
Genes, MHC Class I / immunology*
H-2 Antigens / biosynthesis,  genetics,  metabolism,  physiology
Killer Cells, Natural / cytology,  immunology*,  metabolism
Liver / cytology,  immunology,  metabolism
Lymph Nodes / cytology,  immunology,  metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Promoter Regions, Genetic / immunology*
Spleen / cytology,  immunology,  metabolism
T-Lymphocyte Subsets / cytology,  immunology*,  metabolism
Thymus Gland / cytology,  immunology,  metabolism
Transgenes / immunology
Grant Support
ID/Acronym/Agency:
AI43407/AI/NIAID NIH HHS; R01 AI043407/AI/NIAID NIH HHS; R01 AI043407-07/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD1; 0/Antigens, CD1d; 0/Epitopes, T-Lymphocyte; 0/H-2 Antigens; 0/H-2Kb protein, mouse

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