Document Detail


Expression of C-type natriuretic peptide and of its receptor NPR-B in normal and failing heart.
MedLine Citation:
PMID:  18848850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
C-type natriuretic peptide (CNP) was recently found in the myocardium, but possible insights into differences between atrium and ventricle production are so far lacking. Our aim was to evaluate, in an experimental model of pacing-induced heart failure (HF), plasma and tissue levels of CNP and mRNA expression of the peptide and of its specific receptor, NPR-B. Cardiac tissue was collected from male adult minipigs without (control, n=5) and with pacing-induced HF (n=5). Blood samples were collected at baseline and after pacing (10 min, 1, 2, 3 weeks). CNP in plasma and in cardiac extracts was determined by a radioimmunoassay, while the expression of mRNA by real time PCR. Compared to control, plasma CNP was increased after 1 week of pacing stress (36.9+/-10.4 pg/ml vs.16.7+/-1.1, p=0.013, mean+/-S.E.M.). As to myocardial extract, at baseline, CNP was found in all cardiac chambers and its content was 10-fold higher in atria than in ventricles (RA: 13.7+/-1.9 pg/mg protein; LA: 8.7+/-3.8; RV: 1.07+/-0.33; LV: 0.93+/-0.17). At 3 weeks of pacing, myocardial levels of CNP in left ventricle were higher than in controls (15.8+/-9.9 pg/mg protein vs. 0.9+/-0.17, p=0.01). CNP gene expression was observed in controls and at 3 weeks of pacing. NPR-B gene expression was found in all cardiac regions analyzed, and a down-regulation was observed in ventricles after HF. The co-localization of the CNP system and NPR-B suggests a possible role of CNP in HF and may prompt novel therapeutical strategies.
Authors:
Silvia Del Ry; Manuela Cabiati; Vincenzo Lionetti; Michele Emdin; Fabio A Recchia; Daniela Giannessi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-20
Journal Detail:
Title:  Peptides     Volume:  29     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2208-15     Citation Subset:  IM    
Affiliation:
CNR Institute of Clinical Physiology and Fondazione G. Monasterio, Laboratory of Cardiovascular Biochemistry, Pisa, Italy. delry@ifc.cnr.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiac Pacing, Artificial / methods
Heart Failure / metabolism*
Male
Myocardium / metabolism*
Natriuretic Peptide, C-Type / biosynthesis*
Receptors, Atrial Natriuretic Factor / biosynthesis*
Swine
Swine, Miniature
Chemical
Reg. No./Substance:
127869-51-6/Natriuretic Peptide, C-Type; EC 4.6.1.2/Receptors, Atrial Natriuretic Factor; EC 4.6.1.2/atrial natriuretic factor receptor B

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