| Expression and Biochemical Characterization of Acidic Phospholipase A(2)I from Agkistrodon acutus. | |
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MedLine Citation:
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PMID: 12114959 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A cDNA encoding acidic phospholipase A(2)I(A.aAPLA(2)I)from Agkistrodon acutus was inserted into a bacterial expression vector and effectively expressed in E.coli RR1. The protein was produced as insoluble inclusion bodies. After partial purification by washing the inclusion bodies with Triton X-100, denaturing and refolding, the renatured recombinant protein was purified by FPLC column Superose(TM)12. The enzymatic acti-vity and platelet aggregation inhibiting effect of the expressed A.aAPLA(2)I is close to those of denatured-refolded native acidic PLA(2) from Agkistrodon halys Pallas, and has the same hemolytic activity as denatured-refolded basic phospholipase A(2) from Agkistrodon halys Pallas. The roles of various amino acid residues in the enzymatic activity and pharmacological activities of phospholipase A2 are discussed. |
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Authors:
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Xiao-Long Liu; Xiang-Fu Wu; Ming-De Cai; Yuan-Cong Zhou |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Volume: 31 ISSN: 0582-9879 ISO Abbreviation: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Publication Date: 1999 |
Date Detail:
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Created Date: 2002-Jul-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 20730160R Medline TA: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) Country: - |
Other Details:
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Languages: ENG Pagination: 499-503 Citation Subset: - |
Affiliation:
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Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhouyc@sunm.shcnc.ac.cn |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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