Document Detail


Expression and Biochemical Characterization of Acidic Phospholipase A(2)I from Agkistrodon acutus.
MedLine Citation:
PMID:  12114959     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A cDNA encoding acidic phospholipase A(2)I(A.aAPLA(2)I)from Agkistrodon acutus was inserted into a bacterial expression vector and effectively expressed in E.coli RR1. The protein was produced as insoluble inclusion bodies. After partial purification by washing the inclusion bodies with Triton X-100, denaturing and refolding, the renatured recombinant protein was purified by FPLC column Superose(TM)12. The enzymatic acti-vity and platelet aggregation inhibiting effect of the expressed A.aAPLA(2)I is close to those of denatured-refolded native acidic PLA(2) from Agkistrodon halys Pallas, and has the same hemolytic activity as denatured-refolded basic phospholipase A(2) from Agkistrodon halys Pallas. The roles of various amino acid residues in the enzymatic activity and pharmacological activities of phospholipase A2 are discussed.
Authors:
Xiao-Long Liu; Xiang-Fu Wu; Ming-De Cai; Yuan-Cong Zhou
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  31     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  1999  
Date Detail:
Created Date:  2002-Jul-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  499-503     Citation Subset:  -    
Affiliation:
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhouyc@sunm.shcnc.ac.cn
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