Document Detail


Expression of Bcl-2 and Bax in the human corpus luteum during the menstrual cycle and in early pregnancy: regulation by human chorionic gonadotropin.
MedLine Citation:
PMID:  11095483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the relationship between apoptosis and the Bcl-2/ Bax system in the human corpus luteum (CL), the frequency of apoptosis and expression of Bcl-2 and Bax were examined in the CL during the menstrual cycle and in early pregnancy. In situ analysis of DNA fragmentation showed that the number of apoptotic cells was much greater in the regressing CL than that in the midluteal phase CL, whereas there were almost no apoptotic cells in the CL of early pregnancy. Immunohistochemistry revealed that Bcl-2 expression was observed in the luteal cells in the midluteal phase and early pregnancy, but not in the regressing CL. In contrast, Bax immunostaining was observed in the regressing CL, but not in the midluteal phase and early pregnancy. bcl-2 messenger ribonucleic acid (mRNA) levels in the CL during the menstrual cycle were highest in the midluteal phase and lowest in the regressing CL. In the CL of early pregnancy, bcl-2 mRNA levels were significantly higher than those in the midluteal phase. In contrast, bax mRNA levels were highest in the regressing CL and remarkably low in the CL of early pregnancy. Western blot analyses revealed that Bcl-2 expression was significantly lower in the regressing CL than in the midluteal phase and early pregnancy, and that Bax expression was, in contrast, significantly higher in the regressing CL than in the midluteal phase and was remarkably low in the CL of early pregnancy. When corpora lutea of the midluteal phase were incubated with hCG, hCG significantly increased the mRNA and protein levels of Bcl-2 and significantly decreased those of Bax. In conclusion, Bcl-2 and Bax may play important roles in the regulation of the life span of the human CL by controlling the rate of apoptosis. hCG may act to prolong the life span of the CL by increasing Bcl-2 expression and decreasing Bax expression when pregnancy occurs.
Authors:
N Sugino; T Suzuki; S Kashida; A Karube; S Takiguchi; H Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  85     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-11-29     Completed Date:  2000-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4379-86     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Ube, Japan. obgyn@po.cc.yamaguchi-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Apoptosis*
Chorionic Gonadotropin / pharmacology,  physiology*
Corpus Luteum / cytology,  pathology,  physiology*
Female
Gene Expression Regulation* / drug effects
Humans
Leiomyoma / genetics,  pathology,  surgery
Menstrual Cycle / physiology*
Middle Aged
Pregnancy / physiology*
Proto-Oncogene Proteins / analysis,  genetics*
Proto-Oncogene Proteins c-bcl-2 / analysis,  genetics*
Uterine Cervical Neoplasms / genetics,  pathology,  surgery
Uterine Neoplasms / genetics,  pathology,  surgery
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/BAX protein, human; 0/Chorionic Gonadotropin; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein

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