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Expression of A and B types of monoamine oxidase in neuroblastoma hybrid cells.
MedLine Citation:
PMID:  20487817     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Monoamine oxidase (MAO) activity towards kynuramine as substrate was measured in 6 hybrid cells derived by fusion of neuroblastoma and glioma, liver or brain cells, and was compared with that of parental or non-parental clones. Activities varied from the lowest level of less than 0.15 pmol/min/mg protein in a neuroblastoma clone NB2A to the highest level of 127 pmol/min/mg protein in NCB20 mouse neuroblastoma x Chinese hamster embryo brain hybrid cells. The relative proportions of A and B types of MAO activities were determined in homogenates of each cell line by inhibition curves with clorgyline and deprenyl. Although the A type activity was found in all cell lines measured, MAO A was predominant in 9 clones, except for NCB20 hybrid cells, N4G-B-a neuroblastoma x glioma hybrid cells, and G8-1 myoblast. The ratio of type A/type B activity in NCB20, N4G-B-a and G8-1 cells was 20/80, 75/25 and 95/5, respectively. The results suggest that NCB20 cells are highly enriched in MAO type B, and that the NCB20 cell is an excellent model for studying the type B activity found in the brain in vivo.
Authors:
T Nagatsu; T Nakano; T Kato; H Higashida
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemistry international     Volume:  3     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1981  
Date Detail:
Created Date:  2010-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  137-42     Citation Subset:  -    
Affiliation:
Laboratory of Cell Physiology, Department of Life Chemistry Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama 227 Japan.
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