Document Detail


Expression of aryl hydrocarbon receptor in human placentas and fetal tissues.
MedLine Citation:
PMID:  20354149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, mediates many biological processes, including fetal development. In this study, we examined AhR protein expression in human placentas from normal (N) and severe preeclamptic (sPE) pregnancies, as well as human fetal tissues from the second trimester of pregnancy, using immunohistochemistry and/or Western blot analysis. In the placentas, the AhR immunoreactivity was present primarily in syncytiotrophoblasts. The AhR staining was also seen in endothelium of large blood vessels in villi and endothelium of umbilical cord arteries and veins. No difference in AhR protein levels was found between N and sPE placentas. In fetal tissues, the AhR immunoreactivity was localized in lung, kidney, esophagus, pancreas, liver, testicle, thymus gland, retina, and choroid, mainly in epithelial cells, whereas it was absent in heart, brain, sclera, and thoracic aorta. These findings suggest that the AhR plays a critical role in syncytiotrophoblasts of human placentas and epithelium of many fetal organs. These data also imply that human placentas and those fetal organs with high AhR expression (e.g., lung, kidney, liver, pancreas, and thymus gland) during fetal development are highly susceptible to environmental toxicants such as dioxin.
Authors:
Yi-zhou Jiang; Kai Wang; Roy Fang; Jing Zheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-30
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  58     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-21     Completed Date:  2010-08-17     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  679-85     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, University of Wisconsin, Madison, WI 53715, USA.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Female
Fetus / metabolism*,  ultrastructure
Gene Expression Regulation, Developmental*
Humans
Immunohistochemistry
Placenta / metabolism*,  ultrastructure
Pregnancy
Receptors, Aryl Hydrocarbon / genetics*,  metabolism
Grant Support
ID/Acronym/Agency:
HD-38843/HD/NICHD NIH HHS; HL-64703/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Aryl Hydrocarbon
Comments/Corrections

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