Document Detail


The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells.
MedLine Citation:
PMID:  19932086     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apolipoprotein B mRNA is edited at cytidine 6666 in the enterocytes lining the small intestine of all mammals; converting a CAA codon to a UAA stop codon. The conversion is approximately 80% efficient in this tissue and leads to the expression of the truncated protein, ApoB48, essential for secretion of dietary lipid as chylomicrons. Caco-2 cell raft cultures have been used as an in vitro model for the induction of editing activity during human small intestinal cell differentiation. This induction of apoB mRNA editing has been ascribed to the expression of APOBEC-1. In agreement our data demonstrated differentiation-dependent induction of expression of the editing enzyme APOBEC-1 and in addition we show alternative splicing of the essential auxiliary factor ACF. However, transfection of these editing factors in undifferentiated proliferating Caco-2 cells was not sufficient to induce robust apoB mRNA editing activity. Only differentiation of Caco-2 cells could induce more physiological like levels of apoB mRNA editing. The data suggested that additional regulatory mechanism(s) were induced by differentiation that controlled the functional activity of editing factors.
Authors:
Chad A Galloway; Harold C Smith
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-11-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  391     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-03-15     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  659-63     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing*
Apolipoprotein B-48 / biosynthesis,  genetics
Apolipoproteins B / genetics
Caco-2 Cells
Cell Differentiation / genetics*
Cytidine Deaminase / genetics*
Enterocytes / cytology,  metabolism
Humans
Intestine, Small / cytology*,  enzymology
RNA Editing / genetics*
RNA-Binding Proteins / genetics*
Grant Support
ID/Acronym/Agency:
DK43739/DK/NIDDK NIH HHS; R01 DK043739/DK/NIDDK NIH HHS; R01 DK043739-07/DK/NIDDK NIH HHS; R01 DK043739-07S1/DK/NIDDK NIH HHS; R01 DK043739-08A2/DK/NIDDK NIH HHS; R01 DK043739-09/DK/NIDDK NIH HHS; R01 DK043739-10/DK/NIDDK NIH HHS; R01 DK043739-11/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/A1CF protein, human; 0/Apolipoprotein B-48; 0/Apolipoproteins B; 0/RNA-Binding Proteins; EC 3.5.4.5/Cytidine Deaminase; EC 3.5.4.5/apolipoprotein B mRNA editing enzyme
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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