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Expression analysis of desmosomal components of the novel canine epidermal keratinocyte cell line (MSCEK).
MedLine Citation:
PMID:  20543532     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A novel canine epidermal keratinocyte cell line, MSCEK, was developed from skin of a healthy dog. The aim of this study was to determine its expression of desmosomal components and to evaluate its use as a detection tool for circulating autoantibodies in canine pemphigus. Immunofluorescence and western blotting analyses revealed that MSCEK expresses desmoglein (Dsg) 1, Dsg2, Dsg3, desmoplakin, plakoglobin and cytokeratins. Moreover, positive fluorescent reactions on the surface of MSCEK cells were observed when the cells were incubated with sera obtained from four dogs diagnosed with pemphigus complex. These findings indicate that MSCEK should be a useful tool for future research to characterize circulating autoantibodies that recognize desmosomal components in dogs with pemphigus.
Authors:
Kaori Ide; Waka Yamamoto; Masafumi Sato; Atsushi Yabuzoe; Toshiroh Iwasaki; Koji Nishifuji
Publication Detail:
Type:  Journal Article     Date:  2010-06-11
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  72     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-12-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1479-82     Citation Subset:  IM    
Affiliation:
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
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