Document Detail


Expression of the ammonia transporter family member, Rh B Glycoprotein, in the human kidney.
MedLine Citation:
PMID:  23324176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ammonia transporter family member, Rh B Glycoprotein (RhBG/Rhbg), is essential for ammonia transport by the rodent kidney, but in the human kidney mRNA but not protein expression has been reported. Because ammonia transport is fundamental for acid-base homeostasis, the current study addressed RhBG expression in the human kidney. Two distinct RhBG mRNA sequences have been reported, with different numbers of consecutive cytosines at nt1265 and thus encoding different carboxy-tails. Sequencing the region of difference in both human kidney and liver mRNA showed eight sequential cytosines, not seven as in some reports. Knowing the correct mRNA sequence for RhBG, we then assessed RhBG protein expression using antibodies against the correct amino acid sequence. Immunoblot analysis demonstrated RhBG protein expression in human kidney and immunohistochemistry identified basolateral RhBG in connecting segment (CNT) and the cortical and outer medullary collecting ducts. Colocalization of RhBG with multiple cell-specific markers demonstrated that that CNT cells and collecting duct type A intercalated cells express high levels of RhBG, and type B intercalated cells and principal cells do not express detectable RhBG. Thus, these studies identify the correct mRNA and thus protein sequence for human RhBG and show that the human kidney expresses basolateral RhBG protein in CNT, type A intercalated cells, and non-A, non-B cells. We conclude that RhBG can mediate an important role in human renal ammonia transport.
Authors:
Ki-Hwan Han; Hyun-Wook Lee; Mary E Handlogten; Florence Whitehill; Gunars Osis; Byron P Croker; William L Clapp; Jill W Verlander; I David Weiner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-16
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  304     ISSN:  1522-1466     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-02     Completed Date:  2013-05-23     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F972-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Ammonia / metabolism
Animals
Base Sequence
Glycoproteins / biosynthesis*,  genetics,  immunology
Humans
Kidney / metabolism
Kidney Tubules, Collecting / metabolism*
Liver / metabolism
Membrane Transport Proteins / biosynthesis*,  genetics,  immunology
Mice
Mice, Knockout
Molecular Sequence Data
RNA, Messenger / analysis,  metabolism
Sequence Alignment
Grant Support
ID/Acronym/Agency:
DK-045788/DK/NIDDK NIH HHS; I01 BX000818/BX/BLRD VA; R01 DK045788/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Membrane Transport Proteins; 0/RHBG protein, human; 0/RNA, Messenger; 0/RhBG protein, mouse; 7664-41-7/Ammonia
Comments/Corrections

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