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Expression and activity of matrix metalloproteinases in the uterus of bitches after spontaneous and induced abortion.
MedLine Citation:
PMID:  20477983     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Aim of this study was to determine the intrauterine activity of matrix metalloproteinases (MMP)-2 and -9 after cessation of the local effect of progesterone. For this purpose, pregnancy was terminated in 10 bitches at mid-gestation with the progesterone receptor antagonist aglepristone (10 mg/kg body weight, sc, Alizine(®) ; Virbac, France) at two subsequent days (group IRA = induced resorption/abortion). The IRA group was divided into two subgroups (Group I, n = 5, days 25-35 of pregnancy; group II, n = 5, days 36-45). Five further bitches were introduced with beginning abortion (group SRA = spontaneous resorption/abortion). Seven healthy bitches between day 25 and 45 of gestation served as controls. After ovariohysterectomy at the end of abortion and between days 25 and 45 of gestation, respectively, the distribution and activity of collagenases were investigated by immunohistochemistry and gelatin zymography. At placental sites, MMP-2 activity in the endometrium was significantly lower in IRA groups than in the SRA group (33.7 ± 11.8% and 39.3 ± 5.4% vs 52.2 ± 10.2%, p < 0.05); however, MMP-2 expression was lowest in the control group (control: 21.4 ± 6.3%; p < 0.01) and similarly in the myometrium (controls: 13.1 ± 2.5%; p < 0.05). MMP-9 activity was also lower in the endometrium and myometrium of the control group in comparison to SRA and IRA groups (11.8 ± 3.2%; p < 0.01 and 28.4 ± 32.8%; p < 0.05). At interplacental sites, the amount of active collagenases in the myometrium was significantly lower in the control group. It is concluded that the blockade of the biological progesterone effect was associated with an increase in activity of both collagenases.
Authors:
H Kanca; I Walter; I Miller; S Schäfer-Somi; H Izgur; S Aslan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  46     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  197-204     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, University of Ankara, Ankara, Turkey Institute of Macro- and Microanatomy, University of Veterinary Science, Veterinärplatz 1, Vienna, Austria Institute of Medical Chemistry, University of Veterinary Medicine Vienna, Austria EU Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Science, Veterinärplatz 1, Vienna, Austria.
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