| Expression of the AML-1 oncogene shortens the G(1) phase of the cell cycle. | |
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MedLine Citation:
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PMID: 10652337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle. |
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Authors:
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D K Strom; J Nip; J J Westendorf; B Linggi; B Lutterbach; J R Downing; N Lenny; S W Hiebert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 275 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-03-02 Completed Date: 2000-03-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3438-45 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / genetics* Cell Line Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins* Flow Cytometry G1 Phase / genetics* Gene Expression Regulation* Humans Mice Proto-Oncogene Proteins / genetics Transcription Factors / genetics* Transfection |
| Grant Support | |
ID/Acronym/Agency:
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R01 AG13726/AG/NIA NIH HHS; R01-CA64140/CA/NCI NIH HHS; R01-CA77274/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Core Binding Factor Alpha 2 Subunit; 0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/RUNX1 protein, human; 0/Runx1 protein, mouse; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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