Document Detail

Expression of 72-kDa gelatinase (matrix metalloproteinase-2) in the developing mouse craniofacial complex.
MedLine Citation:
PMID:  9134100     Owner:  NLM     Status:  MEDLINE    
Tissue remodelling is an important feature during embryogenesis. Although the matrix metalloproteinases are believed to participate in these processes, the relation between matrix metalloproteinases and tissue remodelling during craniofacial morphogenesis remains unclear. The purpose of the study was to look for the presence of enzymes involved in extracellular matrix degradation during craniofacial morphogenesis. Protein expression of the matrix metalloproteinase, 72-kDa gelatinase (matrix metalloproteinase-2, gelatinase A, 72-kDa type IV collagenase) was studied by gelatine zymography and by indirect immunofluorescence with conventional and confocal microscopy. In the anterior region of the developing mouse face, 72-kDa gelatinase was labelled mainly in the tips and peripheral regions of the nasal and facial prominences. Upon contact and fusion of the prominences, the staining was intensely localized to the zone of the fusion and the tips and peripheral regions of the nasal prominences and the maxilla. The labelling of 72-kDa gelatinase was also present in the peripheral regions of the mandible, second branchial arch, and the face around the developing eye. However, during lens vesicle formation, the staining of 72-kDa gelatinase was absent in the invaginated lens ectoderm. After the lens had completely detached from the surface ectoderm, the staining was resumed in the corneal epithelium and mesenchyme. Gelatine zymography was used to confirm the presence of active and latent 72-kDa gelatinase in the developing mouse craniofacial complex. Collectively, these data indicate that 72-kDa gelatinase may play a significant part in localized tissue remodelling during craniofacial morphogenesis and the aberrant expression or function of the enzyme could be involved in causing facial abnormalities.
A Iamaroon; U M Wallon; C M Overall; V M Diewert
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  41     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-07-03     Completed Date:  1997-07-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1109-19     Citation Subset:  D; IM    
Department of Odontology and Oral Pathology, Faculty of Dentistry, ChiangMai University, Thailand.
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MeSH Terms
Branchial Region / embryology,  enzymology
Coloring Agents / diagnostic use
Cornea / embryology,  enzymology
Ectoderm / enzymology
Electrophoresis, Polyacrylamide Gel
Epithelium / embryology,  enzymology
Extracellular Matrix / enzymology
Eye / embryology,  enzymology
Facial Bones / embryology*,  enzymology
Fluorescent Antibody Technique, Indirect
Gelatinases / analysis,  genetics*
Gene Expression*
Lens, Crystalline / embryology,  enzymology
Mandible / embryology
Matrix Metalloproteinase 2
Maxilla / embryology,  enzymology
Mesoderm / enzymology
Metalloendopeptidases / analysis,  genetics*
Mice, Inbred Strains
Microscopy, Confocal
Morphogenesis / genetics
Nose / embryology,  enzymology
Skull / embryology*,  enzymology
Reg. No./Substance:
0/Coloring Agents; EC 3.4.24.-/Gelatinases; EC 3.4.24.-/Metalloendopeptidases; EC Metalloproteinase 2

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