| Expression of a 48-kilodalton growth-associated protein in the goldfish retina. | |
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MedLine Citation:
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PMID: 3794726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One of the most striking molecular correlates of optic nerve regeneration in the goldfish is the increased labeling of a 48 kilodalton (kD) acidic protein that is conveyed to the developing nerve endings from the retina by rapid axonal transport. The present study examined the biosynthesis and molecular characteristics of this protein. Retinas derived either from intact controls or from goldfish undergoing optic nerve regeneration (10-14 days postcrush) were pulse-labeled with [3H]proline or [35S]methionine, followed by subcellular fractionation and analysis of protein synthesis patterns by two-dimensional gel electrophoresis and fluorography. Synthesis of the 48-kD acidic protein (termed here GAP-48) was detected only in retinas that were undergoing axonal regeneration. Pulse-chase labeling experiments demonstrated that the protein undergoes a post-translational modification that requires 15-20 min. This processing could be selectively blocked by tunicamycin, an inhibitor of protein N-glycosylation. The protein was also found to incorporate low levels of phosphate in vitro. Thus, the differential appearance of GAP-48 in regenerating axons might be regulated either at the level of gene expression or by selective posttranslational processing in retinal ganglion cells. By the criteria of molecular weight, isoelectric point, anomalous migration properties on sodium dodecyl sulfate-polyacrylamide gels, phosphorylation, subcellular distribution, and the pattern of digestion products generated by Staphylococcus aureus V8 protease, GAP-48 appears to be equivalent to the B-50 (F-1) phosphoprotein of the mammalian brain. |
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Authors:
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N I Perrone-Bizzozero; L I Benowitz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 48 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1987 Feb |
Date Detail:
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Created Date: 1987-02-19 Completed Date: 1987-02-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 644-52 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Eye Proteins / biosynthesis* Goldfish Growth Substances / biosynthesis* Nerve Regeneration Optic Nerve / physiology Retina / metabolism* Time Factors Tunicamycin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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EY 05690/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Eye Proteins; 0/Growth Substances; 0/growth-associated retinal protein 48; 11089-65-9/Tunicamycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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