Document Detail

Expression of a 33-kDa antigen Tm 1 on lymphocyte surface after modulation of cell surface antigens by IgM monoclonal antibody.
MedLine Citation:
PMID:  2963858     Owner:  NLM     Status:  MEDLINE    
The mAb Tm 1 was obtained from a fusion of SP2/O tumor cells with spleen cells from CF1 mouse immunized with T cells modulated by an IgM anti-CD3 mAb.mAb Tm 1 reacted with IgM anti-CD3 modulated T cells (66.6%) but not with unmodulated T cells (4.4%). Tm 1 was not expressed on T cells modulated with either IgG2a or IgG1 anti-CD3 mAb. Immunoprecipitation from 125I-labeled CD3-modulated T cells showed that Tm 1 Ag is a single polypeptide of 33 kDa under reducing and nonreducing conditions. Kinetic studies revealed that Tm 1 was detectable on T cells 10 min after incubation and maximally expressed after 4 h of incubation with IgM anti-CD3 mAb. CD3 expression was markedly modulated by this anti-CD3 mAb after the same period of incubation. Studies with cycloheximide revealed that Tm 1 expression on T cells does not require new protein synthesis. Tm 1 expression persisted long after CD3-reexpression 24 h later. Tm 1 was present on a small fraction of circulating T cells, B cells, and monocytes and absent from granulocytes, platelets, E, and thymocytes. Tm 1 was not expressed on T cells after various activation stimuli but was expressed on B cells upon activation. Additional studies indicate that IgM mAb against other T cell differentiation Ag and IgM mAb against B cell Ag also lead to the expression of Tm 1 on these cells. Thus, modulation of surface Ag by IgM mAb externalizes this cytoplasmic Ag. However, one exception has been noted. Purified mAb Tm 1 was not mitogenic and was unable to block either the T cell proliferation induced by 12-O-tetradecanoyl phorbol-13-acetate plus anti-CD3 mAb and other T cell stimuli, or the B cell proliferation induced by B cell mitogens. The role of Tm 1 on lymphocyte function remains to be determined.
T Hara; L K Jung; S M Fu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  140     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-04-05     Completed Date:  1988-04-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1028-33     Citation Subset:  AIM; IM    
Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City.
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MeSH Terms
Antibodies, Anti-Idiotypic*
Antibodies, Monoclonal / immunology*
Antigens, CD3
Antigens, Differentiation / analysis*,  immunology
Antigens, Differentiation, T-Lymphocyte / immunology
Antigens, Neoplasm / analysis
Cycloheximide / pharmacology
Immunoglobulin G / immunology
Immunoglobulin M / immunology*
Leukemia, Experimental / immunology
Lymphocyte Activation
T-Lymphocytes / immunology*
Tumor Cells, Cultured / immunology
Grant Support
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Antigens, Differentiation; 0/Antigens, Differentiation, T-Lymphocyte; 0/Antigens, Neoplasm; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/anti-IgM; 66-81-9/Cycloheximide

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