Document Detail


Expression of 11beta-hydroxysteroid-dehydrogenase 2 in Sertoli cells of boar testes.
MedLine Citation:
PMID:  17555866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
11beta-Hydroxysteroid-dehydrogenase 2 (11beta-HSD2) activity occurs in boar testes but it is not known which cell types express 11beta-HSD2 mRNA and protein. Therefore, testes samples were taken from mature boars. For immunocytochemistry and Western blot pig-specific antibodies were raised against a 10-amino acid peptide corresponding to amino acids 391-400 of the coding sequence. Quantitative PCR was performed in testis homogenates and additionally RT-PCR in samples collected by UV-single cell microdissection. Data show that in interstitial tissue 11beta-HSD2 is expressed in Leydig cells and additionally in blood capillaries. In tubuli, 11beta-HSD2 primarily is formed in Sertoli cells whereas occurrence in spermatogonia could not be definitely proven. Because glucocorticoid receptors were never found in boar Leydig cells, it is concluded that the expression of 11beta-HSD2 in several types of cells forms consecutive lines of defense to protect spermatogonia against glucocorticoid-induced apoptosis.
Authors:
Rolf Claus; Markus Lacorn; Harald Welter; Oksana Lekhkota; Nina Messe; Anna Wagner; Martin Bergmann
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Publication Detail:
Type:  Journal Article     Date:  2007-05-03
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  272     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  86-92     Citation Subset:  IM    
Affiliation:
Institut für Tierhaltung und Tierzüchtung, Universität Hohenheim, Garbenstr. 17, 70599 Stuttgart, Germany. thsekret@uni-hohenheim.de
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MeSH Terms
Descriptor/Qualifier:
11-beta-Hydroxysteroid Dehydrogenase Type 2 / genetics*,  immunology,  metabolism*
Animals
Antibody Formation
Blotting, Western
Gene Expression
Immunohistochemistry
Male
Microdissection
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
Swine*
Testis / chemistry,  enzymology,  metabolism*
Chemical
Reg. No./Substance:
EC 1.1.1.146/11-beta-Hydroxysteroid Dehydrogenase Type 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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