Document Detail


Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library.
MedLine Citation:
PMID:  1639417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expressed sequence tags (ESTs) provide useful molecular landmarks for physical mapping and identify the position of an expressed region in the genome. The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of ESTs should reduce the repetitive isolation of constitutively expressed sequences. We report here the sequence tags from the 3'-end region of 58 new directionally cloned cDNAs from a subtracted human retinal pigment epithelium (RPE) cell line library. Eight of the cDNAs have been assigned to human chromosomes using PCR-based EST assays. Chromosomal mapping of subtracted RPE cDNA clones may also help in identifying candidate genes for inherited eye diseases.
Authors:
L Gieser; A Swaroop
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  873-6     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Kellogg Eye Center, University of Michigan, Ann Arbor 48105.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M91216;  M91217;  M91218;  M91219;  M91220;  M91221;  M91222;  M91387;  M91388;  M91389;  M91390;  M91391;  M91392;  M91393;  M91394
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA / genetics*
Gene Expression
Genomic Library
Humans
Molecular Sequence Data
Pigment Epithelium of Eye / metabolism*
Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
EY-07961/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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