| Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of beta-endorphin in ventricular-cisternally-perfused rats. | |
| | |
MedLine Citation:
|
PMID: 19747467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have previously shown that the antinociceptive effect of nitrous oxide (N(2)O) in the rat hot plate test is sensitive to antagonism by antisera against the endogenous opioid peptide beta-endorphin. Moreover, N(2)O-induced antinociception is reduced by inhibition of nitric oxide (NO) production in the brain. To test the hypothesis that N(2)O might stimulate an NO-dependent neuronal release of beta-endorphin, we conducted a ventricular-cisternal perfusion with artificial cerebrospinal fluid (aCSF) in urethane-anesthetized Sprague-Dawley rats. Ten-minute fractions of aCSF perfusate were collected from separate groups of room air-exposed rats, N(2)O-exposed rats, and L-NAME-pretreated, N(2)O-exposed rats; they were then analyzed for their content of NO metabolites and beta-endorphin. Compared to room air control, exposure to 70% N(2)O increased perfusate levels of the NO metabolites nitrite and nitrate as well as beta-endorphin. Pretreatment of rats with L-N(G)-nitro arginine methyl ester, an inhibitor of NO synthase, prevented the N(2)O-induced increases in nitrite, nitrate and beta-endorphin. These findings demonstrate in an in vivo rat model that N(2)O may stimulate an NO-dependent neuronal release of beta-endorphin. |
| | |
Authors:
|
Lisa M Zelinski; Yusuke Ohgami; Raymond M Quock |
Related Documents
:
|
8790587 - Transglomerular transport of deae dextran in the isolated perfused kidney. 2352157 - Recovery of rat nasal mucosa from the effects of aminopeptidase inhibitors. 3753457 - Differential effect of glucagon on gluconeogenesis in periportal and pericentral region... 2359297 - Amino acid metabolism in isolated perfused rat liver. 431767 - Ultrastructural changes of oligodendroglia and myelin sheaths induced by ethidium bromide. 620717 - Activation of the carcinogen n-hydroxy-2-acetylaminofluorene by rat mammary peroxidase. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-09-09 |
Journal Detail:
|
Title: Brain research Volume: 1300 ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-10-27 Completed Date: 2010-01-05 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 37-40 Citation Subset: IM |
Affiliation:
|
Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analgesics, Non-Narcotic
/
administration & dosage Analysis of Variance Animals Catheters, Indwelling Enzyme Inhibitors / pharmacology Enzyme-Linked Immunosorbent Assay Male NG-Nitroarginine Methyl Ester / pharmacology Neurons / metabolism* Nitrates / metabolism Nitrites / metabolism Nitrous Oxide / administration & dosage*, metabolism Rats Rats, Sprague-Dawley beta-Endorphin / cerebrospinal fluid* |
| Grant Support | |
ID/Acronym/Agency:
|
GM-77153/GM/NIGMS NIH HHS; R15 GM077153-01A1/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Analgesics, Non-Narcotic; 0/Enzyme Inhibitors; 0/Nitrates; 0/Nitrites; 10024-97-2/Nitrous Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 60617-12-1/beta-Endorphin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neonatal administration of a vaccine preservative, thimerosal, produces lasting impairment of nocice...
Next Document: Inhibition of cerebral ischemia/reperfusion-induced injury by adenovirus expressed C-terminal amino ...