| Exposure to hyperbaric oxygen induces cell cycle perturbation in prostate cancer cells. | |
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MedLine Citation:
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PMID: 10475264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle synchronization of tumor cells by exposure to hyperbaric oxygenation (HBO) may increase the efficacy of chemotherapy or radiation by placing cells into a chemosensitive portion of the cycle. The purpose of the current study was to examine oxygen pressure-dependent relationships with respect to the cell cycle in prostate tumor cells in vitro. LNCaP cells were grown in an incubator at 21% O2 and then exposed to 100% oxygen at pressures up to 6 atmospheres (atm) for 1.5 h. Cells were then returned to the incubator and evaluated for DNA content by propidium iodide and new DNA synthesis with a pulse-chase experiment. Cell cycle effects were evaluated by flow cytometry. Exposure to HBO increased the percentage of cells synthesizing new DNA in a dose-dependent fashion: 0 atm, 44%; 6 atm, 65%. Cells that synthesize new DNA accumulate in G2/M as a function of partial pressure of oxygen. These results suggest that HBO induces cells to enter the cell cycle and accumulate in G2/M. Cell cycle synchronization and entry of senescent cells into the cell cycle suggest that HBO may be a useful adjuvant to chemotherapy or radiation in the treatment of prostate cancer. There are two potential mechanisms of action that may make HBO efficacious in the treatment of prostate cancer. HBO may potentiate cancer chemotherapeutic agents that cause damage to DNA during DNA synthesis or HBO may inhibit cell division causing accumulation in G2/M. |
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Authors:
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J E Kalns; E H Piepmeier |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 35 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-09-20 Completed Date: 1999-09-20 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 98-101 Citation Subset: IM |
Affiliation:
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Davis Hyperbaric Laboratory, Brooks Air Force Base, San Antonio, Texas 78235-5119, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Atmospheric Pressure* Cell Cycle* DNA, Neoplasm / analysis, biosynthesis G2 Phase Humans Male Mitosis Oxygen / administration & dosage* Propidium Prostatic Neoplasms / pathology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 36015-30-2/Propidium; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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