Document Detail


Exposure to fatty acids modulates interferon production by intraepithelial lymphocytes.
MedLine Citation:
PMID:  12644315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intraepithelial lymphocytes (IELs) play important roles in intestinal mucosal immunity. Although fatty acids are known to modulate the functions of immune effector cells, there has been no information about how fat exposure affects immunological function of IELs. In this study, we examined how fatty acids of various chain lengths modulate the production of interferon (IFN)-gamma by IELs stimulated with T-cell receptor (TCR) or interleukin (IL)-12/IL-18. IELs isolated from the small intestine of BALB/c mice were stimulated with plate-coated anti-CD3 monoclonal antibody (mAb) or IL-12/IL-18. They were coincubated in microtiter plates for 3 days with various concentrations of fatty acid micelles. We used arachidonic acid, linoleic acid, and oleic acid as long-chain fatty acids, and used octanoic acid as a medium-chain fatty acid. IFN-gamma in the supernatants were measured by ELISA, and the expression of IFN-gamma mRNA in IELs was determined by RT-PCR. Significant production of IFN-gamma from IELs was observed after anti-CD3 mAb stimulation. The combination of IL-12 and IL-18 induced significant levels of IFN-gamma production without TCR stimulation. Increased IFN-gamma mRNA was also observed after anti-CD3 or IL-12/IL-18 stimulation. Long-chain fatty acids dose-dependently inhibited the stimulated-IFN-gamma production at concentrations greater than 10 micro M, but the medium-chain fatty acid did not cause any significant changes in IFN-gamma production. IFN-gamma production from gammadelta IELs was very low compared with alphabeta IELs, however, both populations showed similar attenuating patterns when treated with long-chain fatty acids. There is a possibility that the exposure of IELs to intraluminal fatty acids significantly modifies the immune function of intestinal mucosa.
Authors:
Yuriko Hara; Soichiro Miura; Shunsuke Komoto; Toshiaki Inamura; Seiichiro Koseki; Chikako Watanabe; Ryota Hokari; Yoshikazu Tsuzuki; Takashi Ogino; Hiroshi Nagata; Satoshi Hachimura; Shuichi Kaminogawa; Hiromasa Ishii
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  86     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-19     Completed Date:  2004-01-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  139-48     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. miura@me.ndmc.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology
Antigens, CD3 / immunology
Fatty Acids / pharmacology*
Female
Interferon-gamma / biosynthesis*
Interleukin-12 / pharmacology
Interleukin-18 / pharmacology
Intestinal Mucosa / cytology,  immunology*
Lymphocyte Activation
Lymphocytes / cytology,  drug effects,  immunology*
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Fatty Acids; 0/Interleukin-18; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma

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