Document Detail


Exposure to dimethyl sulfoxide at 37 degrees C prior to freezing significantly improves the recovery of cryopreserved hybridoma cells.
MedLine Citation:
PMID:  11888222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Standard cryopreservation protocols recommend the use of dimethyl sulfoxide (Me2SO) at moderate temperatures only (room temperature, 4 degrees C) due to its toxicity which appears to be potentiated by warm temperatures. In the present study, we asked whether a transient increase in temperature during membrane sealing of cryovials affects the cell viability. We show here that the cell viability of hybridoma cells and Schwann cells was not reduced following membrane sealing of cryotubes. On the contrary, incubation of cells at 37 degrees C in Me2SO-containing medium prior to freezing significantly stimulated the viability of cryopreserved hybridoma cells, whereas the viability of Schwann cells remained unaltered. We conclude that the exposure of cells to Me2SO at elevated temperatures does not necessarily reduce cell viability and that contrary to this, cell type-specific, beneficial effects of Me2SO could be observed.
Authors:
K Wewetzer; K Dilmaghani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  43     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2002-03-12     Completed Date:  2002-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-92     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Elsevier Science (USA).
Affiliation:
Center of Anatomy OE4140, Hannover Medical School, Carl-Neuberg-Str.1, D-30625 Hannover, Germany. wewetzer.konstantin@mh-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents*
Dimethyl Sulfoxide*
Hybridomas* / cytology
Schwann Cells / cytology
Temperature
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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