| Exposure to bioaccumulative organochlorine compounds alters adipogenesis, fatty acid uptake, and adipokine production in NIH3T3-L1 cells. | |
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MedLine Citation:
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PMID: 21044676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exposure to the organochlorine compounds p,p'-dichlorodiphenyldichloroethylene (DDE) and oxychlordane have been associated with an increased prevalence of diabetes. Although the exact etiology of diabetes, especially type 2 diabetes, is not known, it is thought that adipose dysfunction plays a vital role in the progression of this disease. Thus, the present study examined whether exposure to these bioaccumulative compounds promotes adipocyte dysfunction including alterations in adipogenesis, fatty acid storage, and adipokine production within the adipocyte. We employed the NIH3T3-L1 cell line as a model for adipogenesis and mature adipocyte function. Exposure to DDE or oxychlordane prior to and throughout differentiation did not affect adipogenesis. In mature NIH3T3-L1 adipocytes, exposure to oxychlordane, DDE, or dieldrin had no effect on insulin-stimulated fatty acid uptake but did increase basal fatty acid uptake over a 24 h period. There was no observed effect of exposure to these compounds on lipolysis. Exposure to DDE significantly increased the release of leptin, resistin, and adiponectin from mature adipocytes with corresponding increases in expression of resistin and adiponectin. Taken together, the current data suggest that exposure to these compounds, especially DDE, may promote some aspects of adipocyte dysfunction that are commonly associated with obesity and type 2 diabetes. |
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Authors:
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George Howell; Lauren Mangum |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-31 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 25 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-12-28 Completed Date: 2011-05-17 Revised Date: 2012-04-09 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 394-402 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Center for Environmental Health Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA. howell@cvm.msstate.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes, White
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drug effects*,
metabolism* Adipogenesis / drug effects* Adipokines / genetics, metabolism* Adiponectin / genetics, metabolism Animals Biological Transport / drug effects Chlordan / analogs & derivatives, toxicity Dichlorodiphenyl Dichloroethylene / toxicity Dieldrin / toxicity Fatty Acids / metabolism* Gene Expression Regulation / drug effects Hydrocarbons, Chlorinated / toxicity* Insulin / metabolism Leptin / genetics, metabolism Lipolysis / drug effects Mice NIH 3T3 Cells Pesticides / toxicity* RNA, Messenger / metabolism Resistin / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1K01ES019182/ES/NIEHS NIH HHS; 1P20-RR17661/RR/NCRR NIH HHS; 1R15ES019742/ES/NIEHS NIH HHS; K01 ES019182-01/ES/NIEHS NIH HHS; K01 ES019182-02/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adipokines; 0/Adiponectin; 0/Adipoq protein, mouse; 0/Fatty Acids; 0/Hydrocarbons, Chlorinated; 0/Insulin; 0/Leptin; 0/Pesticides; 0/RNA, Messenger; 0/Resistin; 0/Retn protein, mouse; 12789-03-6/Chlordan; 27304-13-8/oxychlordane; 60-57-1/Dieldrin; 72-55-9/Dichlorodiphenyl Dichloroethylene |
| Comments/Corrections | |
Erratum In:
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Toxicol In Vitro. 2011 Oct;25(7):1507 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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