| Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells. | |
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MedLine Citation:
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PMID: 14603534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been recently established that low-frequency electromagnetic field (EMFs) exposure induces biological changes and could be associated with increased incidence of cancer, while the issue remains unresolved as to whether high-frequency EMFs can have hazardous effect on health. Epidemiological studies on association between childhood cancers, particularly leukemia and brain cancer, and exposure to low- and high-frequency EMF suggested an etiological role of EMFs in inducing adverse health effects. To investigate whether exposure to high-frequency EMFs could affect in vitro cell survival, we cultured acute T-lymphoblastoid leukemia cells (CCRF-CEM) in the presence of unmodulated 900 MHz EMF, generated by a transverse electromagnetic (TEM) cell, at various exposure times. We evaluated the effects of high-frequency EMF on cell growth rate and apoptosis induction, by cell viability (MTT) test, FACS analysis and DNA ladder, and we investigated pro-apoptotic and pro-survival signaling pathways possibly involved as a function of exposure time by Western blot analysis. At short exposure times (2-12 h), unmodulated 900 MHz EMF induced DNA breaks and early activation of both p53-dependent and -independent apoptotic pathways while longer continuous exposure (24-48 h) determined silencing of pro-apoptotic signals and activation of genes involved in both intracellular (Bcl-2) and extracellular (Ras and Akt1) pro-survival signaling. Overall our results indicate that exposure to 900 MHz continuous wave, after inducing an early self-defense response triggered by DNA damage, could confer to the survivor CCRF-CEM cells a further advantage to survive and proliferate. |
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Authors:
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F Marinelli; D La Sala; G Cicciotti; L Cattini; C Trimarchi; S Putti; A Zamparelli; L Giuliani; G Tomassetti; Caterina Cinti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 198 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2003-11-06 Completed Date: 2004-02-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 324-32 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Wiley-Liss, Inc. |
Affiliation:
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Institute for Organ Transplantation and Immunocytology, ITOI-CNR, Bologna unit, c/o IOR, Bologna, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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radiation effects* Blotting, Western Cell Cycle / genetics, radiation effects Cell Division / radiation effects Electromagnetic Fields / adverse effects* Gene Expression / radiation effects* Humans Leukocytes / radiation effects* Time Factors Tumor Cells, Cultured Tumor Suppressor Protein p53 / radiation effects |
| Chemical | |
Reg. No./Substance:
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0/Tumor Suppressor Protein p53 |
| Comments/Corrections | |
Erratum In:
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J Cell Physiol. 2004 Mar;198(3):479-80 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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