Document Detail


Exposure-time-dependent effects on the relative potencies and additivity of PAHs in the AH receptor-based H4IIE-luc bioassay.
MedLine Citation:
PMID:  22328298     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the present study, relative potency factors (REPs) of 16 individual polycyclic aromatic hydrocarbons (PAHs) were investigated using the H4IIE-luc bioassay. Exposure time-dependent effects on the REPs were examined using 24, 48, and 72 h of exposure. Seven different mixtures of PAHs were tested for additivity at an exposure time of 24 h. Three of the PAH mixtures were also studied at 48 and 72 h of exposure. The mixture toxicities were predicted using the REP concept and the concentration addition (CA) model. Relative potency factor values investigated in the present study were similar to those reported in earlier studies. Declining REPs with an increasing exposure time were shown for all PAHs, indicating that this bioassay approach could be developed to assess the persistency of aryl hydrocarbon receptor (AhR) agonistic PAHs and in the risk assessment of complex PAH mixtures. The results from the mixture studies indicated that additive interactions of PAHs are time dependent. Generally, 48- and 72-h exposures resulted in biological effects that were similar to the CA and REP model predictions, while these models tended to underestimate the effect, to some extent, in the 24-h exposure, at least for the mixtures containing two to four PAHs. Thus, it cannot be ruled out that in the 24-h exposures, the tested PAH mixtures had slight synergistic effects. Further research is needed to identify and test additional AhR activating PAHs and investigate whether the effects in the H4IIE-luc bioassay are additive for more complex samples containing both PAHs and other AhR-activating contaminants. Also, the observed superinduction of luciferase by PAH-mixes warrants studies of whether this also can occur for relevant AhR-mediated endpoints in vivo. Environ. Toxicol. Chem. © 2012 SETAC.
Authors:
Maria Larsson; Dan Orbe; Magnus Engwall
Related Documents :
16800618 - Generalization of the friction theory for viscosity modeling.
40248 - Optimum probabilistic processing in colour perception. i. colour discrimination.
22324398 - Predicting concentrations of organic chemicals in fish by using toxicokinetic models.
10927288 - Reverse monte carlo modelling of neutron powder diffraction data.
22687708 - Evaluating the efficiency of municipalities in collecting and processing municipal soli...
16364968 - Accuracy of coalescent likelihood estimates: do we need more sites, more sequences, or ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-10
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  -     ISSN:  1552-8618     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 SETAC.
Affiliation:
Man-Technology-Environment Research Centre, School of Science and Technology, Örebro University, Örebro, Sweden. maria.larsson@oru.se.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Skin tissue generation by laser cell printing.
Next Document:  Determination of circulating tumor cells for prediction of recurrent colorectal cancer progression.