Document Detail

Exposure of mice to cigarette smoke and/or light causes DNA alterations in heart and aorta.
MedLine Citation:
PMID:  18640134     Owner:  NLM     Status:  MEDLINE    
Cigarette smoke (CS) is a major risk factor for cardiovascular diseases, cancer, and other chronic degenerative diseases. UV-containing light is the most ubiquitous DNA-damaging agent existing in nature, but its possible role in cardiovascular diseases had never been suspected before, although it is known that mortality for cardiovascular diseases is increased during periods with high temperature and solar irradiation. We evaluated whether exposure of Swiss CD-1 mice to environmental CS (ECS) and UV-C-covered halogen quartz lamps, either individually or in combination, can cause DNA damage in heart and aorta cells. Nucleotide alterations were evaluated by (32)P postlabeling methods and by HPLC-electrochemical detection. The whole-body exposure of mice to ECS considerably increased the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and of bulky DNA adducts in both heart and aorta. Surprisingly, even exposure to a light that simulated solar irradiation induced oxidatively generated damage in both tissues. The genotoxic effects of UV light in internal organs is tentatively amenable to formation of unidentified long-lived mutagenic products in the skin of irradiated mice. Nucleotide alterations were even more pronounced when the mice were exposed to smoke and/or light during the first 5 weeks of life rather than during adulthood for an equivalent period of time. Although the pathogenetic meaning is uncertain, DNA damage in heart and aorta may tentatively be related to cardiomyopathies and to the atherogenesis process, respectively.
Alberto Izzotti; Francesco D'Agostini; Roumen Balansky; Paolo Degan; Tanya M Pennisi; Vernon E Steele; Silvio De Flora
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-07-03
Journal Detail:
Title:  Mutation research     Volume:  644     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-25     Completed Date:  2008-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  38-42     Citation Subset:  IM    
Department of Health Sciences, University of Genoa, Genoa, Italy.
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MeSH Terms
Animals, Newborn
Aorta, Thoracic / metabolism*,  radiation effects
DNA Adducts / metabolism,  radiation effects
DNA Damage*
DNA Glycosylases / genetics
Deoxyguanosine / analogs & derivatives,  metabolism
GTP Phosphohydrolases / genetics
Heart / radiation effects
Myocardium / metabolism*
Tobacco Smoke Pollution / adverse effects*
Transcription, Genetic
Ultraviolet Rays / adverse effects*
Grant Support
Reg. No./Substance:
0/DNA Adducts; 0/Tobacco Smoke Pollution; 88847-89-6/8-oxo-7-hydrodeoxyguanosine; 961-07-9/Deoxyguanosine; EC 3.2.2.-/DNA Glycosylases; EC 3.2.2.-/Ogg1 protein, mouse; EC 3.6.1.-/GTP Phosphohydrolases

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