| Exposure of human diploid fibroblasts to hypoxia extends proliferative life span. | |
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MedLine Citation:
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PMID: 18056950 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal human mitotic cells do not proliferate indefinitely in culture but undergo a limited number of divisions and progressively reach a state of irreversible growth arrest, a process termed replicative senescence. Hypoxia is a situation of reduced oxygen concentration that relates to many physiological and pathophysiological conditions. In the current study we investigated the effects of oxygen concentration, in both normoxic and hypoxic conditions, on the proliferative capacity, cell viability, oxidative status, and protein expression of human embryonic diploid fibroblasts. Maintenance of WI38 and IMR90 cells in 1.5% or 3% O(2) concentration significantly delayed the appearance of replicative senescence compared to cells grown in 20% O(2), induced the hypoxia-inducible factor-1alpha, and resulted in reduced expression levels of the key cell cycle modulators, namely p21 and p16. Moreover, cell exposure to short-term hypoxia affected their response to several cytotoxic agents, whereas adaptation to the hypoxic environment prior to the treatment had no impact on cell viability. These findings clearly identify that oxygen concentration plays a crucial role in regulating cellular life span of normal human cells. |
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Authors:
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Efthymios Poulios; Ioannis P Trougakos; Niki Chondrogianni; Efstathios S Gonos |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 1119 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
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Languages: eng Pagination: 9-19 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular & Cellular Aging, Institute of Biological Research & Biotechnology, National Hellenic Research Foundation, Athens 11635, Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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physiology Cell Aging / physiology* Cell Cycle / physiology* Cell Hypoxia / physiology Cell Line Cell Survival / physiology Diploidy Embryo, Mammalian / cytology, physiology* Fibroblasts / cytology, physiology* Gene Expression Regulation / physiology* Humans Oxidative Stress / physiology Oxygen / metabolism* |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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