| Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1{alpha} and airway responsiveness to ACh. | |
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MedLine Citation:
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PMID: 17660326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Airway smooth muscle (ASM) cells are characterized by phenotypic plasticity and can switch between differentiated and proliferative phenotypes. In rabbit tracheal ASM cells that had been differentiated in vitro by serum starvation, readdition of FBS caused initiation of proliferation and induction of nuclear and transcriptionally active hypoxia-inducible factor (HIF)-1alpha. In addition, FBS stimulated the induction of HIF-1alpha by the hypoxia mimetic cobalt. Treatment with actinomycin D, cycloheximide, the phosphatidylinositol 3-kinase inhibitors LY-294002 and wortmannin or the reactive oxygen species scavenger diphenyleneiodonium inhibited the FBS-dependent induction of HIF-1alpha. These data indicate that, in differentiated ASM cells, FBS upregulates HIF-1alpha by a transcription-, translation-, phosphatidylinositol 3-kinase-, and reactive oxygen species-dependent mechanism. Interestingly, addition of FBS and cobalt also induced HIF-1alpha in organ cultures of rabbit trachea strips and synergistically increased their contractile response to ACh, suggesting that HIF-1alpha might be implicated in airway hypercontractility. |
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Authors:
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Georgia Chachami; Apostolia Hatziefthimiou; Panagiotis Liakos; Maria G Ioannou; Georgios K Koukoulis; Sofia Bonanou; Paschalis-Adam Molyvdas; George Simos; Efrosyni Paraskeva |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-27 |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: 293 ISSN: 1040-0605 ISO Abbreviation: Am. J. Physiol. Lung Cell Mol. Physiol. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-09 Completed Date: 2007-11-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: United States |
Other Details:
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Languages: eng Pagination: L913-22 Citation Subset: IM |
Affiliation:
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Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Thessaly, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Acetylcholine / pharmacology* Animals Cattle / embryology Cell Differentiation Cells, Cultured Cobalt / pharmacology Drug Stability Drug Synergism Fetal Blood Hypoxia-Inducible Factor 1 / biosynthesis*, chemistry Muscle, Smooth / metabolism Myocytes, Smooth Muscle / cytology*, metabolism* Myosin Heavy Chains / metabolism Protein Biosynthesis / physiology Rabbits Reactive Oxygen Species / metabolism Serum* Signal Transduction / physiology Trachea / cytology*, drug effects, metabolism* Transcription, Genetic / physiology |
| Chemical | |
Reg. No./Substance:
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0/Hypoxia-Inducible Factor 1; 0/Myosin Heavy Chains; 0/Reactive Oxygen Species; 51-84-3/Acetylcholine; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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