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Exposure of Bacillus subtilis to low pressure (5 kPa) induces several global regulons including the sigB-mediated General Stress Response.
MedLine Citation:
PMID:  24878601     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Studies of how microorganisms respond to pressure have been limited mostly to the extreme high pressures of the deep sea (i.e., the piezosphere). In contrast-despite the fact that the growth of most bacteria is inhibited at pressures below ∼2.5 kPa-little is known of microbial responses to low pressure (LP). To study the global LP response, we performed transcription microarrays on Bacillus subtilis cells grown under normal atmospheric pressure (∼101 kPa) vs. a near-inhibitory LP (5 kPa), equivalent to an altitude of ∼20 km. Microarray analysis revealed altered levels of 363 transcripts belonging to several global regulons (AbrB, CcpA, CodY, Fur, IolR, ResD, Rok, SigH, Spo0A). Notably, the highest number of up-regulated genes, 86, belonged to the SigB-mediated General Stress Response (GSR) regulon. Up-regulation of the GSR by LP was confirmed by monitoring the expression of the sigB-dependent ctc-lacZ reporter fusion. Measuring transcriptome changes resulting from exposure of bacterial cells to LP reveals insights into cellular processes that may respond to LP exposure.
Authors:
Samantha M Waters; José A Robles-Martínez; Wayne L Nicholson
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-30
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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