Document Detail

Export of microRNAs and microRNA-protective protein by mammalian cells.
MedLine Citation:
PMID:  20615901     Owner:  NLM     Status:  MEDLINE    
The discovery of microRNAs (miRNAs) as a new class of regulators of gene expression has triggered an explosion of research activities, but has left many unanswered questions about how this regulation functions and how it is integrated with other regulatory mechanisms. A number of miRNAs have been found to be present in plasma and other body fluids of humans and mice in surprisingly high concentrations. This observation was unexpected in two respects: first, the fact that these molecules are present at all outside the cell at significant concentrations and second, that these molecules appear to be stable outside of the cell. In light of this it has been suggested that the biological function of miRNAs may also extend outside of the cell and mediate cell-cell communication. We report here that after serum deprivation several human cell lines tested promptly export a substantial amount of miRNAs into the culture medium and the export process is largely energy dependent. The exported miRNAs are found both within and outside of the 16.5 and 120 K centrifugation pellets which contain most of the known cell-derived vesicles, the microvesicles and exosomes. We have identified some candidate proteins involved in this system, and one of these proteins may also play a role in protecting extracellular miRNAs from degradation. Our results point to a hitherto unrecognized and uncharacterized miRNA trafficking system in mammalian cells that is consistent with the cell-cell communication hypothesis.
Kai Wang; Shile Zhang; Jessica Weber; David Baxter; David J Galas
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-07-07
Journal Detail:
Title:  Nucleic acids research     Volume:  38     ISSN:  1362-4962     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-12     Completed Date:  2011-01-04     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  7248-59     Citation Subset:  IM    
Institute for Systems Biology, 1441 N. 34th Street, Seattle, WA 98103, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism
Cell Communication
Cell Line
Culture Media, Serum-Free
MicroRNAs / biosynthesis,  metabolism*
Nuclear Proteins / metabolism*
RNA Stability
RNA Transport
RNA-Binding Proteins / metabolism*
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/MicroRNAs; 0/Nuclear Proteins; 0/RNA-Binding Proteins; 117896-08-9/nucleophosmin; 56-65-5/Adenosine Triphosphate

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