Document Detail


Exponential-phase glycogen recycling is essential for growth of Mycobacterium smegmatis.
MedLine Citation:
PMID:  10542168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial glycogen is a polyglucose storage compound that is thought to prolong viability during stationary phase. However, a specific role for glycogen has not been determined. We have characterized SMEG53, a temperature-sensitive mutant of Mycobacterium smegmatis that contains a mutation in glgE, encoding a putative glucanase. This mutation causes exponentially growing SMEG53 cells to stop growing at 42 degrees C in response to high levels of glycogen accumulation. The mutation in glgE is also associated with an altered growth rate and colony morphology at permissive temperatures; the severity of these phenotypes correlates with the amount of glycogen accumulated by the mutant. Suppression of the temperature-sensitive phenotype, via a decrease in glycogen accumulation, is mediated by growth in certain media or multicopy expression of garA. The function of GarA is unknown, but the presence of a forkhead-associated domain suggests that this protein is a member of a serine-threonine kinase signal transduction pathway. Our results suggest that in M. smegmatis glycogen is continuously synthesized and then degraded by GlgE throughout exponential growth. In turn, this constant recycling of glycogen controls the downstream availability of carbon and energy. Thus, in addition to its conventional storage role, glycogen may also serve as a carbon capacitor for glycolysis during the exponential growth of M. smegmatis.
Authors:
A E Belanger; G F Hatfull
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  181     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-06     Completed Date:  1999-12-06     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6670-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF172946;  AF173844
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Glycogen / metabolism*
Glycoside Hydrolases / chemistry,  genetics*,  metabolism*
Molecular Sequence Data
Mutagenesis
Mycobacterium smegmatis / drug effects,  enzymology*,  growth & development*
Nitrosoguanidines / pharmacology
Polymerase Chain Reaction / methods
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Sequence Analysis, DNA
Temperature
Grant Support
ID/Acronym/Agency:
AI37848/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Nitrosoguanidines; 9005-79-2/Glycogen; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.2.1.-/Glycoside Hydrolases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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