Document Detail


Exploring the yeast acetylome using functional genomics.
MedLine Citation:
PMID:  22579291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysine acetylation is a dynamic posttranslational modification with a well-defined role in regulating histones. The impact of acetylation on other cellular functions remains relatively uncharacterized. We explored the budding yeast acetylome with a functional genomics approach, assessing the effects of gene overexpression in the absence of lysine deacetylases (KDACs). We generated a network of 463 synthetic dosage lethal (SDL) interactions involving class I and II KDACs, revealing many cellular pathways regulated by different KDACs. A biochemical survey of genes interacting with the KDAC RPD3 identified 72 proteins acetylated in vivo. In-depth analysis of one of these proteins, Swi4, revealed a role for acetylation in G1-specific gene expression. Acetylation of Swi4 regulates interaction with its partner Swi6, both components of the SBF transcription factor. This study expands our view of the yeast acetylome, demonstrates the utility of functional genomic screens for exploring enzymatic pathways, and provides functional information that can be mined for future studies.
Authors:
Supipi Kaluarachchi Duffy; Helena Friesen; Anastasia Baryshnikova; Jean-Philippe Lambert; Yolanda T Chong; Daniel Figeys; Brenda Andrews
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  149     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-14     Completed Date:  2012-07-02     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  936-48     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Amino Acid Sequence
DNA-Binding Proteins / chemistry,  metabolism
Gene Deletion
Gene Expression Regulation, Fungal
Genomics*
Histone Deacetylases / metabolism
Histones / metabolism
Molecular Sequence Data
Saccharomyces cerevisiae / genetics*,  metabolism
Saccharomyces cerevisiae Proteins / analysis,  chemistry,  genetics,  metabolism*
Transcription Factors / chemistry,  metabolism
Grant Support
ID/Acronym/Agency:
MOP-102629//Canadian Institutes of Health Research; MOP-11206//Canadian Institutes of Health Research; MOP97939//Canadian Institutes of Health Research; R01 HG005853/HG/NHGRI NIH HHS; R01HG005853-01/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Histones; 0/SWI4 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; EC 3.5.1.-/RPD3 protein, S cerevisiae; EC 3.5.1.98/Histone Deacetylases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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