| Exploring permeability of Escherichia coli competence using quantum dots as fluorescent probes. | |
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MedLine Citation:
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PMID: 15571775 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Though people had recognized the pivotal function of CaCl(2) during DNA transformation into Escherichia coli, the mechanism of divalent Ca(2+) cation inducing E. coli competence development is still unknowable. Quantum dots (QDs), as a new fluorescent probe, being applied in biology research, had aroused great interest. We explored the penetrability of E. coli competent cells membrane using QDs and proved directly that competent cells were more permeable than that of noncompetent. The results are significant on understanding the problems of the microbiological genetics. |
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Authors:
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Li Wenhua; Xie Haiyan; Xie Zhixiong; Ou Jianhong; Chen Xiangdong; Shen Ping |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 61 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-12-01 Completed Date: 2005-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 265-70 Citation Subset: IM |
Affiliation:
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College of Life Sciences, Wuhan University, Wuhan 430072, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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pharmacology* Cell Membrane Permeability / drug effects* Escherichia coli / cytology, drug effects, genetics*, metabolism* Fluorescent Dyes Microscopy, Fluorescence / methods* Plasmids / administration & dosage, pharmacokinetics* Quantum Dots* Spectrometry, Fluorescence / methods Transformation, Bacterial / genetics |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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