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Exploring the membrane mechanism of the bioactive peptaibol ampullosporin a using lipid monolayers and supported biomimetic membranes.
MedLine Citation:
PMID:  21403824     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Ampullosporin A is an antimicrobial, neuroleptic peptaibol, the behavior of which was investigated in different membrane mimetic environments made of egg yolk L-α-phosphatidylcholine. In monolayers, the peptaibol adopted a mixed α/3(10)-helical structure with an in-plane orientation. The binding step was followed by the peptide insertion into the lipid monolayer core. The relevance of the inner lipid leaflet nature was studied by comparing ampullosporin binding on a hybrid bilayer, in which this leaflet was a rigid alkane layer, and on supported fluid lipid bilayers. The membrane binding was examined by surface plasmon resonance spectroscopy and the effect on lipid dynamics was explored using fluorescence recovery after photobleaching. In the absence of voltage and at low concentration, ampullosporin A substantially adsorbed onto lipid surfaces and its interaction with biomimetic models was strongly modified depending on the inner leaflet structure. At high concentration, ampullosporin A addition led to the lipid bilayers disruption.
Authors:
Marguerita Eid; Sonia Rippa; Sabine Castano; Bernard Desbat; Joël Chopineau; Claire Rossi; Laure Béven
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Publication Detail:
Type:  Journal Article     Date:  2011-02-17
Journal Detail:
Title:  Journal of biophysics (Hindawi Publishing Corporation : Online)     Volume:  2010     ISSN:  1687-8019     ISO Abbreviation:  J Biophys     Publication Date:  2010  
Date Detail:
Created Date:  2011-03-15     Completed Date:  2011-07-14     Revised Date:  2011-07-26    
Medline Journal Info:
Nlm Unique ID:  101519205     Medline TA:  J Biophys     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  179641     Citation Subset:  -    
Affiliation:
UMR 6022 CNRS Génie Enzymatique et Cellulaire, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France.
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