Document Detail


Exploring the binding sites of anti-infliximab antibodies in pediatric patients with rheumatic diseases treated with infliximab.
MedLine Citation:
PMID:  21131896     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Over the past decade, the treatment of a variety of immune-mediated diseases has improved greatly due to the introduction of biologics for therapies in cases that are nonresponsive to traditional treatments. However, a side effect not encountered in traditional treatments is the immunogenicity of the biologics themselves. Our aim was to investigate the anti-infliximab-antibody response in pediatric patients receiving infliximab for juvenile idiopathic arthritis and other pediatric rheumatic diseases, with a focus on an analysis of the binding sites of these antibodies. We show that anti-infliximab antibodies developed in 43% of patients receiving infliximab therapy. Neutralization studies showed that in all these patients, the antibodies were directed toward the variable domains of infliximab, as they inhibited binding of infliximab to TNF. A more precise determination of the antibody epitopes using synthetic peptides was not achieved, indicating that all the antibody binding sites were composed of discontinuous segments of infliximab. ABBREVIATIONS::
Authors:
Miha Kosmač; Tadej Avčin; Nataša Toplak; Gabriele Simonini; Rolando Cimaz; Vladka Curin Šerbec
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pediatric research     Volume:  69     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Affiliation:
Department for Production of Diagnostic Reagents and Research [M.K., V.Č.Š.], Blood Transfusion Centre of Slovenia, University of Ljubljana, SI-1000 Ljubljana, Slovenia; Department of Allergology, Rheumatology and Clinical Immunology [T.A., N.T.], University Children's Hospital, University Medical Centre, SI-1000 Ljubljana, Slovenia; Department of Paediatrics [G.S., R.C.], Anna Meyer Children's Hospital, University of Florence, 50139 Florence, Italy.
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