| Exploring leukocyte mitochondrial membrane potential in type 1 diabetes families. | |
| | |
MedLine Citation:
|
PMID: 20963513 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Proper cellular function requires the maintenance of mitochondrial membrane potential (MMP) sustained by the electron transport chain. Mitochondrial dysfunction is believed to play a role in the development of diabetes and diabetic complications possibly because of the active generation of free radicals. Since MMP can be investigated in clinical settings using fluorescent probes and living whole blood cells, mitochondrial membrane alterations have been observed in some chronic disorders. We have used the mitochondrial indicator 5,5',6,6'-tetra chloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) in conjunction with flow cytometry to measure the MMP in peripheral blood granulocytes from type 1 diabetes (T1D) families. The intracellular ROS levels and the respiratory burst activity were also measured. Leukocyte MMP was elevated in 20 T1D patients and their 20 non-diabetic siblings compared with 25 healthy subjects without family history of T1D. Fasting plasma glucose was the only correlate of MMP. If confirmed by further observations, the functional implications of mitochondrial hyperpolarisation (probably different among different cells) will require extensive investigation. |
| | |
Authors:
|
Elena Matteucci; Massimo Ghimenti; Cristina Consani; Maria Chiara Masoni; Ottavio Giampietro |
Related Documents
:
|
15786723 - Prevention of cataract by pyruvate in experimentally diabetic mice. 16524643 - Soluble semicarbazide-sensitive amine oxidase (ssao) activity is related to oxidative s... 22272193 - Sex differences in the association between level of childhood interleukin-6 and insulin... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cell biochemistry and biophysics Volume: 59 ISSN: 1559-0283 ISO Abbreviation: Cell Biochem. Biophys. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9701934 Medline TA: Cell Biochem Biophys Country: United States |
Other Details:
|
Languages: eng Pagination: 121-6 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, University of Pisa, via Roma 67, 56126, Pisa, Italy, ematteuc@int.med.unipi.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical evaluation of targeted arterial infusion of verapamil in the interventional chemotherapy of...
Next Document: Screening and Identification of a Fungal ?-Glucosidase and the Enzymatic Synthesis of Gentiooligosac...