Document Detail


Exploration of self-renewal and pluripotency in ES cells using RNAi.
MedLine Citation:
PMID:  20699150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Embryonic stem cells (ESCs) have the ability to expand indefinitely in vitro and give rise to cells of all three germ layers as well as germ cells. For these reasons, ESCs hold great promise for biomedicine. In order to harness the potential of pluripotent cells, it is necessary to first understand the molecular mechanisms that control the pluripotent state. The discovery of RNA interference has made such functional analysis, even at high(er) throughput, possible. Here, we describe the methods used for high-throughput siRNA screening by high-content microscopy to identify gene products that regulate mouse ESC fate decision. In addition, we will describe the application of lentivirus-based shRNA knockdown to explore or validate the role of candidate genes in ESC pluripotency.
Authors:
Christoph Schaniel; Dung-Fang Lee; Ihor R Lemischka
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  477     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-11     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-65     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryonic Stem Cells / metabolism*
Mice
RNA Interference / physiology*
RNA, Small Interfering / genetics
Chemical
Reg. No./Substance:
0/RNA, Small Interfering

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