| Exploiting the nitrilotriacetic acid moiety for biolabeling with ultrastable perylene dyes. | |
| | |
MedLine Citation:
|
PMID: 18376823 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon binding of the nickel ions. Herein we present the solution and solid phase synthesis of water-soluble perylene(dicarboximide) functionalized with a nitrilotriacetic acid moiety (PDI-NTA). The photophysical properties of PDI-NTA revealed an exceptional photostability and fluorescence quantum yield that remained unchanged upon addition of nickel ions. The F1 complex of F0F1-ATP synthase from Escherichia coli, containing three hexahistidine tags, was labeled and the suitability for site-specific labeling of the new chromophore demonstrated using fluorescence correlation spectroscopy. |
| | |
Authors:
|
Kalina Peneva; Gueorgui Mihov; Andreas Herrmann; Nawid Zarrabi; Michael Börsch; Thomas M Duncan; Klaus Müllen |
Related Documents
:
|
23108453 - An ascorbic acid sensor based on protein-modified au nanoclusters. 16345733 - Mycophenolic acid production by penicillium brevicompactum on solid media. 12617463 - What is the role of alpha-linolenic acid for mammals? |
Publication Detail:
|
Type: Journal Article Date: 2008-04-01 |
Journal Detail:
|
Title: Journal of the American Chemical Society Volume: 130 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-04-18 Completed Date: 2008-07-11 Revised Date: 2008-12-15 |
Medline Journal Info:
|
Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
|
Languages: eng Pagination: 5398-9 Citation Subset: IM |
Affiliation:
|
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphatases
/
metabolism Binding Sites Escherichia coli / enzymology Fluorescence Resonance Energy Transfer Fluorescent Dyes / chemical synthesis* Nickel / chemistry Nitrilotriacetic Acid / chemistry* Perylene / analogs & derivatives, chemical synthesis* Photons Solubility Water / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 139-13-9/Nitrilotriacetic Acid; 198-55-0/Perylene; 7440-02-0/Nickel; 7732-18-5/Water; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Solvothermal synthesis and characterization of two high-nuclearity mixed-valent manganese phosphonat...
Next Document: Modification of fluorophore photophysics through peptide-driven self-assembly.