Document Detail


Exploiting microRNA regulation for genetic engineering.
MedLine Citation:
PMID:  23020307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RNA interference (RNAi) has been a landmark discovery in science. A typical application is to knock down the expression of endogenous genes by delivering small interfering RNA (siRNA) into cells triggering the degradation of complementary mRNA. However, RNAi can also be exploited the other way round: making use of the huge diversity of endogenous microRNAs (miRNA), the expression of exogenously introduced genes tagged with artificial miRNA target sequences can be negatively regulated according to the activity of a given miRNA which can be tissue-, lineage-, activation- or differentiation stage specific. This has significantly expanded the regulatory potential of gene transfer vectors and will benefit both basic science and therapeutic applications. This review briefly introduces the reader to the technical basis for exploiting miRNA regulation, followed by a discussion of specific applications for miRNA-regulated vectors/viruses in basic research, gene- and virotherapy.
Authors:
B Gentner; L Naldini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Tissue antigens     Volume:  80     ISSN:  1399-0039     ISO Abbreviation:  Tissue Antigens     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-04-29     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0331072     Medline TA:  Tissue Antigens     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  393-403     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Expression Regulation
Gene Silencing
Genetic Engineering*
Genetic Vectors
Humans
Mice
MicroRNAs / chemistry,  genetics*,  metabolism
Oncolytic Virotherapy
RNA, Small Interfering / chemistry,  genetics*,  metabolism
Transfection
Transgenes
Viral Tropism
Viruses / genetics
Grant Support
ID/Acronym/Agency:
TGT11D02//Telethon
Chemical
Reg. No./Substance:
0/MicroRNAs; 0/RNA, Small Interfering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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