Document Detail


Exploiting differential surface display of chondroitin sulfate variants for directing neuronal outgrowth.
MedLine Citation:
PMID:  23947484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chondroitin sulfate (CS) proteoglycans (CSPGs) are known to be primary inhibitors of neuronal regeneration at scar sites. However, a variety of CSPGs are also involved in neuronal growth and guidance during other physiological stages. Sulfation patterns of CS chains influence their interactions with various growth factors in the central nervous system (CNS), thus influencing neuronal growth, inhibition, and pathfinding. This report demonstrates the use of differentially sulfated CS chains for neuronal navigation. Surface-immobilized patterns of CS glycosaminoglycan chains were used to determine neuronal preference toward specific sulfations of five CS variants: CS-A, CS-B (dermatan sulfate), CS-C, CS-D, and CS-E. Neurons preferred CS-A, CS-B, and CS-E and avoided CS-C containing lanes. In addition, significant alignment of neurites was observed using underlying lanes containing CS-A, CS-B, and CS-E chains. To utilize differential preference of neurons toward the CS variants, a binary combinations of CS chains were created by backfilling a neuro-preferred CS variant between the microcontact printed lanes of CS-C stripes, which are avoided by neurons. The neuronal outgrowth results demonstrate for the first time that a combination of sulfation variants of CS chains without any protein component of CSPG is sufficient for directing neuronal outgrowth. Biomaterials with surface immobilized GAG chains could find numerous applications as bridging devices for tackling CNS injuries where directional growth of neurons is critical for recovery.
Authors:
Vimal P Swarup; Tony W Hsiao; Jianxing Zhang; Glenn D Prestwich; Balagurunathan Kuberan; Vladimir Hlady
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-08-30
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  135     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-09-11     Completed Date:  2014-04-08     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13488-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Growth Processes / drug effects*
Cells, Cultured
Chondroitin Sulfates / chemistry,  pharmacology*
Molecular Structure
Neurons / cytology*,  drug effects*
Rats
Structure-Activity Relationship
Surface Properties
Grant Support
ID/Acronym/Agency:
R01 DC004336/DC/NIDCD NIH HHS; R01 NS057144/NS/NINDS NIH HHS; R01 NS57144/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
9007-28-7/Chondroitin Sulfates
Comments/Corrections

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