Document Detail


Exploiting cell surface thiols to enhance cellular uptake.
MedLine Citation:
PMID:  22260747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Efficient cellular delivery is one of the key issues that has hampered the therapeutic development of novel synthetic biomolecules such as oligonucleotides, peptides and nanoparticles. The highly specialized cellular plasma membrane specifically internalizes compounds through tightly regulated mechanisms. It is possible to exploit these natural mechanisms of cellular uptake with rationally designed reagents. Here, we discuss how thiol groups (-SH) naturally present on the cell surface (exofacial thiols) can be used to enhance cellular association and internalization of various materials bearing thiol-reactive groups in their structure. We propose that such thiol modifications should be considered in future design of synthetic biomolecules for optimized cellular delivery.
Authors:
Adrian G Torres; Michael J Gait
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-17
Journal Detail:
Title:  Trends in biotechnology     Volume:  30     ISSN:  1879-3096     ISO Abbreviation:  Trends Biotechnol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-26     Completed Date:  2012-07-03     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8310903     Medline TA:  Trends Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-90     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cell Membrane / chemistry*,  metabolism*
Extracellular Space / metabolism*
Models, Biological
Nanoparticles / chemistry
Oligonucleotides / chemistry,  metabolism
Peptides / chemistry,  metabolism
Sulfhydryl Compounds / chemistry*,  metabolism*
Grant Support
ID/Acronym/Agency:
MC_U105178803//Medical Research Council; U105178803//Medical Research Council
Chemical
Reg. No./Substance:
0/Oligonucleotides; 0/Peptides; 0/Sulfhydryl Compounds

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