Document Detail

Exploiting 4-sulphate N-acetyl galactosamine decorated gelatin nanoparticles for effective targeting to professional phagocytes in vitro and in vivo.
MedLine Citation:
PMID:  23020515     Owner:  NLM     Status:  Publisher    
The present study was focused on the development of surface modified gelatin nanoparticles (SGNPs) using novel ligand 4-sulfated N-acetyl galactosamine (4-SO(4)GalNAc) for specific targeting to macrophages. The gelatin has been modified with the potential targeting moiety 4-SO(4)GalNAc, which was further used for the preparation of modified nanoparticles. The nanoparticles have been prepared by two step desolvation method. The SGNPs and unmodified gelatin nanoparticles (GNPs) were loaded with doxorubicin (DxR) and its targeting potential was compared. Developed DxR-loaded SGNPs (DxR-SGNPs) were found to have negative zeta potential (-19.8 ± 0.22 mV) whereas DxR-loaded GNPs (DxR-GNPs) have the positive zeta potential of around +12.2 ± 0.36 mV. The mean particle size of DxR-SGNPs and DxR-GNPs was found to be 283 ± 7 and 134 ± 5 nm, respectively. Flow cytometric data confirmed the enhanced uptake of DxR-SGNPs in J774A.1 and PBMC when compared with DxR-GNPs. Intracellular localization studies indicate that the fluorescence intensity of DxR-SGNPs was significantly higher when compared to DxR-GNPs. DxR-SGNPs rendered significantly higher localization of DxR in liver and spleen as compared to DxR-GNPs after i.v. administration. The study stipulates that 4-SO(4)GalNAc assures for targeting resident macrophages.
Pankaj Dwivedi; Shaswat Kansal; Monika Sharma; Rahul Shukla; Ashwini Verma; Prashant Shukla; Priyanka Tripathi; Pramod Gupta; Deepika Saini; Kiran Khandelwal; Rahul Verma; Anil Kumar Dwivedi; Prabhat Ranjan Mishra
Related Documents :
25463575 - Developing resilience to england's future droughts: time for cap and trade?
11391785 - Molecularly imprinted polymers from nicotinamide and its positional isomers.
11537695 - Minimizing waste polymers in space.
21051635 - Large-area three-dimensional molecular ordering of a polymer brush by one-step processing.
952935 - A proton magnetic resonance study of water in human stratum corneum.
20383295 - Epitaxial structure of (001)- and (111)-oriented perovskite ferrate films grown by puls...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Journal of drug targeting     Volume:  -     ISSN:  1029-2330     ISO Abbreviation:  J Drug Target     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312476     Medline TA:  J Drug Target     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Pharmaceutics Division, CSIR-Central Drug Research Institute , Lucknow , India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evidence for different mechanisms of 'unhooking' for melphalan and cisplatin-induced DNA interstrand...
Next Document:  Enantioselective Baeyer-Villiger Oxidation: Desymmetrization of meso-Cyclic Ketones and Kinetic Reso...