Document Detail

Exploitation of the endocytic pathway by Orientia tsutsugamushi in nonprofessional phagocytes.
MedLine Citation:
PMID:  16790799     Owner:  NLM     Status:  MEDLINE    
Orientia tsutsugamushi, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. We observed the localization of O. tsutsugamushi with clathrin or adaptor protein 2 within 30 min after the infection of nonprofessional phagocytes. We have further confirmed that the infectivity of O. tsutsugamushi is significantly reduced by drugs that block clathrin-mediated endocytosis but not by filipin III, an inhibitor that blocks caveola-mediated endocytosis. In the present study, with a confocal microscope, O. tsutsugamushi was sequentially colocalized with the early and late endosomal markers EEA1 and LAMP2, respectively, within 1 h after infection. The colocalization of O. tsutsugamushi organisms with EEA1 and LAMP2 gradually disappeared until 2 h postinfection, and then free O. tsutsugamushi organisms were found in the cytoplasm. When the acidification of endocytic vesicles was blocked by treating the cells with NH(4)Cl or bafilomycin A, the escape of O. tsutsugamushi organisms from the endocytic pathway was severely impaired, and the infectivity of O. tsutsugamushi was drastically reduced. To our knowledge, this is the first report that the invasion of O. tsutsugamushi is dependent on the clathrin-dependent endocytic pathway and the acidification process of the endocytic vesicles in nonprofessional phagocytes.
Hyuk Chu; Jung-Hee Lee; Seung-Hoon Han; Se-Yoon Kim; Nam-Hyuk Cho; Ik-Sang Kim; Myung-Sik Choi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  74     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-08-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4246-53     Citation Subset:  IM    
Department of Microbiology and Immunology, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, Republic of Korea.
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MeSH Terms
Cell Line
Cell Line, Transformed
Clathrin / physiology
Endocytosis / physiology*
Endothelium, Vascular / cytology,  microbiology
Fibroblasts / microbiology
Macrophages / microbiology*
Orientia tsutsugamushi / physiology*
Signal Transduction / physiology*
Reg. No./Substance:
Erratum In:
Infect Immun. 2006 Sep;74(9):5422

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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